Western Blot (WB)
(Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using S6 Ribosomal Protein R2031-30D (top), Phospho-S6 Ribosomal Protein (Ser235/236) MBS627244 (middle) or Phospho-S6 Ribosomal Protein (Ser240/244) R2031-32 (bottom).)
Rabbit Ribosomal Protein S6 Polyclonal Antibody | anti-Despr_1827 antibody
Ribosomal Protein S6, phosphorylated (Ser235/236)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Ribosomal Protein S6; Polyclonal Antibody; phosphorylated (Ser235/236); Anti -Ribosomal Protein S6; anti-Despr_1827 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of human ribosomal protein S6 only when phosphorylated at Ser235/236 (Mr 32kD). Does not detect ribosomal protein S6 phosphorylated at other sites. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-Despr_1827 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry.
Dilution: Western Blot: 1:1,000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (for IF): 1:50
Flow Cytometry: 1:50
Dilution: Western Blot: 1:1,000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (for IF): 1:50
Flow Cytometry: 1:50
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6 (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using S6 Ribosomal Protein R2031-30D (top), Phospho-S6 Ribosomal Protein (Ser235/236) MBS627244 (middle) or Phospho-S6 Ribosomal Protein (Ser240/244) R2031-32 (bottom).)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using S6 Ribosomal Protein R2031-30D (top), Phospho-S6 Ribosomal Protein (Ser235/236) MBS627244 (middle) or Phospho-S6 Ribosomal Protein (Ser240/244) R2031-32 (bottom).)
Related Product Information for anti-Despr_1827 antibody
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylate the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors
Product Categories/Family for anti-Despr_1827 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
ribosomal protein S6
NCBI Official Symbol
Despr_1827
NCBI Protein Information
ribosomal protein S6
Protein Family
Similar Products
Product Notes
The Despr_1827 (Catalog #AAA627244) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ribosomal Protein S6, phosphorylated (Ser235/236) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ribosomal Protein S6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Dilution: Western Blot: 1:1,000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 Immunocytochemistry (for IF): 1:50 Flow Cytometry: 1:50. Researchers should empirically determine the suitability of the Despr_1827 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribosomal Protein S6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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