Rabbit anti-Human REDD1 Polyclonal Antibody | anti-ddit4 antibody

REDD1 (Regulated in Development and DNA damage responses)

Cat. Num. AAA610486

• Gene Names: ddit4; redd1; rtp801; sb:cb821; zgc:64114
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by peptide affinity chromatography.
• Synonyms: REDD1; Polyclonal Antibody; REDD1 (Regulated in Development and DNA damage responses); Anti -REDD1 (Regulated in Development and DNA damage responses); anti-ddit4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects trans-fected levels of REDD1 protein.
• Purity/Purification: Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-ddit4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) surrounding Asn60 of human REDD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from COS-7 cells, transfected with HA-REDD1 or untransfected (mock), using MBS610486.)

Related Product Information for anti-ddit4 antibody

REDD1 (REgulated in Development and DNA damage responses) expression is induced by hypoxia, cell stress, apoptosis and DNA damage. REDD1 is a transcriptional target of p53 and p63 following DNA damage, and links p63 to Ros (1). REDD1 functions as a negative regulator of mTOR in response to hypoxia in a tuberin-dependent manner (2). Depending on cell context, REDD1 can either be protecting or detrimental for cells under oxidative or ischemic stresses (3).

Product Categories/Family for anti-ddit4 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 329754640
• NCBI GeneID: 378866
• UniProt Accession #: Q7T346
• Molecular Weight: 23,976 Da
• NCBI Official Full Name: regulated in development and DNA damage response 1
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 4
• NCBI Official Symbol: ddit4
• NCBI Official Synonym Symbols: redd1; rtp801; sb:cb821; zgc:64114
• NCBI Protein Information: DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4 protein; regulated in development and DNA damage response 1
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: ddit4
• UniProt Entry Name: DDIT4_DANRE

Uniprot Description

Function: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves ddit4/redd1, akt1, the tsc1-tsc2 complex and the GTPase rheb. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage, via its effect on mTORC1 activity. Plays a role in neuronal differentiation, neuron migration during embryonic brain development and in neuronal cell death

By similarity.

Subcellular location: Cytoplasm

By similarity. Mitochondrion

By similarity.

Sequence similarities: Belongs to the DDIT4 family.