Rabbit anti-Human DDIT4 Polyclonal Antibody | anti-DDIT4 antibody

DDIT4, CT (DDIT4, REDD1, RTP801, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1) (AP)

Cat. Num. AAA6291288

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: DDIT4; Polyclonal Antibody; CT (DDIT4; REDD1; RTP801; DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1) (AP); Protein regulated in development and DNA damage response 1; anti-DDIT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDIT4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DDIT4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 198-227 in the C-terminal region of human DDIT4, UniProt Accession #Q9NX09; Q8VHZ9; Q9D3F7; Q08E62. Species sequence homology: bovine, mouse, rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS643557 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDIT4 gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS643557 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS643557 for immunohistochemistry.)

Related Product Information for anti-DDIT4 antibody

REDD1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS); TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD1. Thus, REDD1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

Product Categories/Family for anti-DDIT4 antibody

Antibodies; Transcription Factors

References

1. Ellisen L.W., Mol. Cell 10:995-1005(2002). 2. Shoshani T., Mol. Cell. Biol. 22:2283-2293(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931.1
• NCBI GenBank Nucleotide #: NM_019058.2
• UniProt Accession #: Q9NX09; Q9H0S3
• Molecular Weight: 25,371 Da
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1
• UniProt Entry Name: DDIT4_HUMAN

Uniprot Description

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; cytoplasm; intracellular; cytosol

Biological Process: neuron differentiation; cell proliferation; negative regulation of TOR signaling pathway; protein complex disassembly; nerve growth factor receptor signaling pathway; neuron migration; response to hypoxia; negative regulation of peptidyl-serine phosphorylation; brain development; negative regulation of glycolysis; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis