Human Keratin 40 (KRT40) ELISA Kit | KRT40 elisa kit

Human Keratin 40 (KRT40) ELISA Kit

Cat. Num. AAA2707294

• Gene Names: KRT40; K40; KA36; CK-40
• Reactivity: Human
• Synonyms: Keratin 40 (KRT40); Human Keratin 40 (KRT40) ELISA Kit; KA36; CK-40; K40; Cytokeratin-40; Type I hair keratin Ka36; KRT40 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KRT4. No significant cross-reactivity or interference between KRT4 and analogues was observed.
• Sequence Length: 431

• Samples: Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KRT4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KRT4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT40 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KRT4 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to KRT4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to KRT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KRT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KRT4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 125490370
• NCBI GeneID: 125115
• NCBI Accession #: NP_872303.2
• NCBI GenBank Nucleotide #: NM_182497.3
• UniProt Accession #: Q6A162; Q6IFU5
• Molecular Weight: 48,139 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 40
• NCBI Official Synonym Full Names: keratin 40
• NCBI Official Symbol: KRT40
• NCBI Official Synonym Symbols: K40; KA36; CK-40
• NCBI Protein Information: keratin, type I cytoskeletal 40
• UniProt Protein Name: Keratin, type I cytoskeletal 40
• Protein Family: Keratin
• UniProt Gene Name: KRT40
• UniProt Synonym Gene Names: KA36; CK-40; K40

NCBI Description

This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009]

Uniprot Description

May play a role in late hair differentiation.