Mouse anti-Human DDIT4 Polyclonal Antibody | anti-DDIT4 antibody

DDIT4 (REDD1, RTP801, DNA Damage-inducible Transcript 4 Protein, HIF-1 Responsive Protein RTP801, Protein Regulated in Development and DNA Damage Response 1, FLJ20500)

Cat. Num. AAA6000808

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DDIT4; Polyclonal Antibody; DDIT4 (REDD1; RTP801; DNA Damage-inducible Transcript 4 Protein; HIF-1 Responsive Protein RTP801; Protein Regulated in Development and DNA Damage Response 1; FLJ20500); Anti -DDIT4 (REDD1; anti-DDIT4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDIT4.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC

• Applicable Applications for anti-DDIT4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human DDIT4, aa1-232 (NP_061931.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DDIT4 expression in transfected 293T cell line by DDIT4 polyclonal antibody. Lane 1: DDIT4 transfected lysate (25.52kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DDIT4 antibody

REDD1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS); TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD1. Thus, REDD1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

Product Categories/Family for anti-DDIT4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 115495515
• NCBI GeneID: 529235
• NCBI Accession #: NP_001069390.1
• NCBI GenBank Nucleotide #: NM_001075922.1
• UniProt Accession #: Q08E62
• Molecular Weight: 24,811 Da
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 4<
• NCBI Official Symbol: DDIT4
• NCBI Protein Information: DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4 protein
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Entry Name: DDIT4_BOVIN

Uniprot Description

Function: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. Required for mTORC1-mediated defense against viral protein synthesis and virus replication

By similarity.

Subunit structure: Monomer. Interacts with BTRC. Identified in a complex with CUL4A, DDB1 and BTRC. Interacts with TXNIP; this inhibits the proteasomal degradation of DDIT4

By similarity.

Subcellular location: Mitochondrion

By similarity. Cytoplasm › cytosol

By similarity.

Post-translational modification: Phosphorylated by GSK3B; this promotes proteasomal degradation

By similarity.Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation

By similarity.

Sequence similarities: Belongs to the DDIT4 family.