Western Blot (WB)
(Anti-RBM34 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:49 kDa Observed band size:55 kDa)
Rabbit anti-Human RBM34 Polyclonal Antibody | anti-RBM34 antibody
Anti-RBM34 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
RBM34; Polyclonal Antibody; Anti-RBM34 Antibody; Rabbit Polyclonal; RBM34 Antibody; Rabbit PAb; Antigen Affinity Purified; RNA binding motif protein 34; Anti-KIAA0117 Antibody; anti-RBM34 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RBM34
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RBM34 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human RBM34 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RBM34 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-RBM34 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:49 kDa Observed band size:55 kDa)
Western Blot (WB)
(Anti-RBM34 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:49 kDa Observed band size:55 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of RBM34 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RBM34 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleolus.)
Immunofluorescence (IF)
(Immunofluorescence staining of RBM34 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RBM34 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleolus.)
Immunoprecipitation (IP)
(RBM34 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-RBM34 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBM34 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa Observed band size :56 kDa)
Immunoprecipitation (IP)
(RBM34 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-RBM34 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RBM34 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa Observed band size :56 kDa)
Related Product Information for anti-RBM34 antibody
RBM34 (RNA Binding Motif Protein 34, also known as KIAA0117) is a Protein Coding gene. The RBM34 gene, located on 1q42.3, is conserved in chimpanzee, Rhesus monkey, dog, mouse, rat, chicken, zebrafish, and frog. This gene encodes a member of the RNA-binding motif family of RNA recognition motif proteins. The encoded protein contains an RNA-binding domain made up of two RNA recognition motif subdomains referred to as RNA recognition motif-1 and RNA recognition motif-2. RBM34 is widely expressed in the testis, lymph node, and other tissues. Alternative splicing results in multiple transcript variants. 235 organisms have orthologs with the human gene RBM34.
References
Li HD, et al. (2015) Functional networks of highest-connected splice isoforms: From the chromosome 17 human proteome project. J Proteome Res 14 (9): 3484-3491.Kartvelishvili A, et al. (2004) Microarray analysis of differentially expressed genes in cells resistant to hiv-1. Immunol Lett 93 (1): 79-86.Clery A, et al. (2008) Rna recognition motifs: Boring? Not quite. Curr Opin Struct Biol 18 (3): 290-298.
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Product Notes
The RBM34 (Catalog #AAA8108837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RBM34 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBM34 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ICC/IF: 1:100-1:500 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the RBM34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBM34, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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