Rabbit CENPO Polyclonal Antibody | anti-CENPO antibody

CENPO Antibody

Cat. Num. AAA150527

• Gene Names: CENPO; CENP-O; MCM21R; ICEN-36
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: CENPO Antibody is affinity chromatography purified via peptide column.
• Synonyms: CENPO; Polyclonal Antibody; CENPO Antibody; CENP-O; MCM21R; ICEN-36; ICEN36; Centromere protein O; Interphase centromere complex protein 36; centromere protein O; anti-CENPO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CENPO Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 300

• Applicable Applications for anti-CENPO antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO.
• Buffer: CENPO Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CENPO antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-CENPO antibody

CENPO Antibody: Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.

NCBI and Uniprot Product Information

• NCBI GI #: 13236565
• NCBI GeneID: 79172
• NCBI Accession #: NP_077298
• NCBI GenBank Nucleotide #: NM_024322.2
• UniProt Accession #: Q9BU64; Q53T55; Q96JV3; B2RDC0; D6W536
• Molecular Weight: 32,989 Da
• NCBI Official Full Name: centromere protein O isoform 1
• NCBI Official Synonym Full Names: centromere protein O
• NCBI Official Symbol: CENPO
• NCBI Official Synonym Symbols: CENP-O; MCM21R; ICEN-36
• NCBI Protein Information: centromere protein O; centromeric protein O; interphase centromere complex protein 36
• UniProt Protein Name: Centromere protein O
• Protein Family: Centromere protein
• UniProt Gene Name: CENPO
• UniProt Synonym Gene Names: ICEN36; MCM21R; CENP-O
• UniProt Entry Name: CENPO_HUMAN

NCBI Description

This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CENPO: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytosol

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle