Rabbit anti-Human RAIDD Polyclonal Antibody | anti-CRADD antibody

RAIDD Antibody

Cat. Num. AAA151340

• Gene Names: CRADD; MRT34; RAIDD
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: RAIDD Antibody is Antibody is DEAE purified.
• Synonyms: RAIDD; Polyclonal Antibody; RAIDD Antibody; MRT34; Death domain-containing protein CRADD; Caspase and RIP adapter with death domain; CASP2 and RIPK1 domain containing adaptor with death domain; anti-CRADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RAIDD Antibody is Antibody is DEAE purified.
• Form/Format: Liquid
• Sequence Length: 199

• Applicable Applications for anti-CRADD antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: RAIDD antibody can be used for detection of RAIDD by Western blot at 1 mug/mL. A 22 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RAIDD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human RAIDD.
• Buffer: RAIDD Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RAIDD antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RAIDD in MCF7 total cell lysate with RAIDD antibody at 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RAIDD in human kidney tissue with RAIDD antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.Green: RAIDD Antibody (1117)Blue: DAPI staining)

Immunocytochemistry (ICC)

(Immunocytochemistry of RAIDD in Hela cells with RAIDD antibody at 10 μg/mL.)

Related Product Information for anti-CRADD antibody

RAIDD Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.

NCBI and Uniprot Product Information

• NCBI GI #: 1785557
• NCBI GeneID: 8738
• UniProt Accession #: P78560; B7Z2Q5
• Molecular Weight: 22 kDa
• NCBI Official Full Name: death adaptor molecule RAIDD
• NCBI Official Synonym Full Names: CASP2 and RIPK1 domain containing adaptor with death domain
• NCBI Official Symbol: CRADD
• NCBI Official Synonym Symbols: MRT34; RAIDD
• NCBI Protein Information: death domain-containing protein CRADD; death adaptor molecule RAIDD; caspase and RIP adaptor with death domain; RIP-associated protein with a death domain; RIP-associated ICH1/CED3-homologous protein with death domain
• UniProt Protein Name: Death domain-containing protein CRADD
• Protein Family: Death domain-containing protein
• UniProt Gene Name: CRADD
• UniProt Synonym Gene Names: RAIDD
• UniProt Entry Name: CRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q21.33-q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding, bridging; protein binding; protease binding

Biological Process: caspase activation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Mental Retardation, Autosomal Recessive 34