Rabbit anti-Human CRADD / RAIDD Polyclonal Antibody | anti-CRADD antibody

Rabbit Polyclonal to Human CRADD / RAIDD

Cat. Num. AAA241265

• Gene Names: CRADD; MRT34; RAIDD
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: CRADD / RAIDD; Polyclonal Antibody; Rabbit Polyclonal to Human CRADD / RAIDD; Anti-CRADD / RAIDD Antibody (aa99-117) IHC-plus; CRADD; Death adaptor molecule RAIDD; RAIDD; MRT34; Human CRADD; anti-CRADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: peptide corresponding to amino acids 99 to 117 of human RAIDD
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 199

• Applicable Applications for anti-CRADD antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: A peptide corresponding to amino acids 99 to 117 of human RAIDD.
• Immunogen Type: Synthetic peptide
• Immunogen: CRADD / RAIDD antibody was raised against a peptide corresponding to amino acids 99 to 117 of human RAIDD.
• Antigen Modification: aa99-117
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-CRADD / RAIDD antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CRADD / RAIDD antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of RAIDD in HeLa cells with RAIDD antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 ug/ml.)

Western Blot (WB)

(Western blot of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.)

Related Product Information for anti-CRADD antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules.

Product Categories/Family for anti-CRADD antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4503031
• NCBI GeneID: 8738
• NCBI Accession #: NP_003796.1
• NCBI GenBank Nucleotide #: NM_003805.3
• UniProt Accession #: P78560; B7Z2Q5
• Molecular Weight: 11,826 Da
• NCBI Official Full Name: death domain-containing protein CRADD
• NCBI Official Synonym Full Names: CASP2 and RIPK1 domain containing adaptor with death domain
• NCBI Official Symbol: CRADD
• NCBI Official Synonym Symbols: MRT34; RAIDD
• NCBI Protein Information: death domain-containing protein CRADD; death adaptor molecule RAIDD; caspase and RIP adaptor with death domain; RIP-associated protein with a death domain; RIP-associated ICH1/CED3-homologous protein with death domain
• UniProt Protein Name: Death domain-containing protein CRADD
• Protein Family: Death domain-containing protein
• UniProt Gene Name: CRADD
• UniProt Synonym Gene Names: RAIDD
• UniProt Entry Name: CRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q21.33-q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding, bridging; protein binding; protease binding

Biological Process: caspase activation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Mental Retardation, Autosomal Recessive 34