CRADD / RAIDD recombinant protein

Recombinant Human CRADD / RAIDD Protein (His tag)

Cat. Num. AAA2545442

• Gene Names: CRADD; MRT34; RAIDD
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CRADD / RAIDD; Recombinant Human CRADD / RAIDD Protein (His tag); MGC9163; RAIDD; CRADD / RAIDD recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH 8.0
• Sequence Length: 199

• Application Notes: The recombinant human CRADD consisting of 209 amino acids and has a calculated molecular mass of 24.1 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CRADD / RAIDD recombinant protein

Background: Death domain-containing protein CRADD, also known as Caspase and RIP adapter with death domain, RIP-associated protein with a death domain, CRADD and RAIDD, is a protein which is constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. CRADD / RAIDD contains one CARD domain and one death domain. CRADD / RAIDD contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2. FADD / MORT1 is a death domain (DD)-containing adaptor / signaling molecule that interacts with the intracellular DD of FAS / APO-I (CD95) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 (Mch5 / MACH / FLICE). CRADD / RAIDD has a dual-domain structure similar to that of FADD. CRADD / RAIDD has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD / RAIDD could play a role in regulating apoptosis in mammalian cells. CRADD / RAIDD is a apoptotic adaptor molecule specific for caspase-2 and FASL / TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD / RAIDD recruits caspase-2 to the TNFR-1 signalling complex.

Description: A DNA sequence encoding the human CRADD (P78560) (Met 1-Glu 199) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 991820347
• NCBI GeneID: 8738
• NCBI Accession #: NP_001307028.1
• NCBI GenBank Nucleotide #: NM_001320099.1
• UniProt Accession #: P78560; B7Z2Q5
• Molecular Weight: 11,826 Da
• NCBI Official Full Name: death domain-containing protein CRADD isoform 1
• NCBI Official Synonym Full Names: CASP2 and RIPK1 domain containing adaptor with death domain
• NCBI Official Symbol: CRADD
• NCBI Official Synonym Symbols: MRT34; RAIDD
• NCBI Protein Information: death domain-containing protein CRADD
• UniProt Protein Name: Death domain-containing protein CRADD
• UniProt Gene Name: CRADD
• UniProt Synonym Gene Names: RAIDD

NCBI Description

This gene encodes a protein containing a death domain (DD) motif. This protein recruits caspase 2/ICH1 to the cell death signal transduction complex, which includes tumor necrosis factor receptor 1 (TNFR1A) and RIPK1/RIP kinase, and acts in promoting apoptosis. A mutation in this gene was associated with mental retardation. A related pseudogene is found on chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protease binding; protein binding; protein binding, bridging

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; induction of apoptosis via death domain receptors; regulation of apoptosis

Disease: Mental Retardation, Autosomal Recessive 34