Western Blot (WB)
(Western blot analysis of NEK8 expression in K562 (A), mouse liver (B) whole cell lysates.)
Rabbit anti-Human, Mouse NEK8 Polyclonal Antibody | anti-NEK8 antibody
Anti-NEK8 Antibody
IF/ICC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of NEK8 expression in K562 (A), mouse liver (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NEK8 expression in K562 (A), mouse liver (B) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of NEK8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of NEK8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Observed MW: 80kDa
NCBI Description
This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008]
Uniprot Description
NEK8: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. May play a role in mitotic regulation.Required for renal tubular integrity.
Protein type: EC 2.7.11.1; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family
Chromosomal Location of Human Ortholog: 17q11.1
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding
Biological Process: organ morphogenesis; mitotic cell cycle; protein amino acid phosphorylation; microtubule-based process
Disease: Renal-hepatic-pancreatic Dysplasia 2; Nephronophthisis 9
Research Articles on NEK8
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Product Notes
The NEK8 nek8 (Catalog #AAA8307413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NEK8 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEK8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the NEK8 nek8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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