Rabbit anti-Human RAD51C Polyclonal Antibody | anti-RAD51C antibody

RAD51C, ID (RAD51C, RAD51L2, DNA repair protein RAD51 homolog 3, RAD51 homolog C, RAD51-like protein 2) (FITC)

Cat. Num. AAA6340023

• Gene Names: RAD51C; FANCO; R51H3; BROVCA3; RAD51L2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAD51C; Polyclonal Antibody; ID (RAD51C; RAD51L2; DNA repair protein RAD51 homolog 3; RAD51 homolog C; RAD51-like protein 2) (FITC); RAD51-like protein 2; anti-RAD51C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 376

• Applicable Applications for anti-RAD51C antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAD51C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the Central region of human RAD51C.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RAD51C Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RAD51C antibody detected the RAD51C protein (arrow).)

Related Product Information for anti-RAD51C antibody

This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. This gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified.

Product Categories/Family for anti-RAD51C antibody

Antibodies; DNA Replication; Repair

References

Zheng, Y., et al. Breast Cancer Res. Treat. (2010). In press: Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Monsees, G.M., et al. Breast Cancer Res. Treat. (2010). In press: Meindl, A., et al. Nat. Genet. 42(5):410-414(2010). Vaz, F., et al. Nat. Genet. 42(5):406-409(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 17402896
• NCBI GeneID: 5889
• NCBI Accession #: NP_478123.1; NP_002867.1
• NCBI GenBank Nucleotide #: NP_478123.1
• UniProt Accession #: O43502
• NCBI Official Full Name: DNA repair protein RAD51 homolog 3 isoform 1
• NCBI Official Synonym Full Names: RAD51 paralog C
• NCBI Official Symbol: RAD51C
• NCBI Official Synonym Symbols: FANCO; R51H3; BROVCA3; RAD51L2
• NCBI Protein Information: DNA repair protein RAD51 homolog 3
• UniProt Protein Name: DNA repair protein RAD51 homolog 3
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51C
• UniProt Synonym Gene Names: RAD51L2; R51H3
• UniProt Entry Name: RA51C_HUMAN

NCBI Description

This gene is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in this gene are associated with Fanconi anemia-like syndrome. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

RAD51C: Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis. Defects in RAD51C are the cause of Fanconi anemia complementation group O (FANCO). It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. Defects in RAD51C are the cause of familial breast- ovarian cancer type 3 (BROVCA3). It is a condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Belongs to the RecA family. RAD51 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: nucleoplasm; mitochondrion; perinuclear region of cytoplasm; cytoplasm; replication fork; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; four-way junction DNA binding; crossover junction endodeoxyribonuclease activity; ATP binding

Biological Process: male meiosis I; meiotic recombination; sister chromatid cohesion; spermatogenesis; female meiosis sister chromatid cohesion; blood coagulation; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; DNA recombination

Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Breast-ovarian Cancer, Familial, Susceptibility To, 3; Fanconi Anemia, Complementation Group O