Mouse ATG5 Monoclonal Antibody | anti-ATG5 antibody

ATG5 Monoclonal Antibody

Cat. Num. AAA2548925

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A Purification
• Synonyms: ATG5; Monoclonal Antibody; ATG5 Monoclonal Antibody; Autophagy protein 5 (APG5-like) (Apoptosis-specific protein); anti-ATG5 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 5D3
• Purity/Purification: Protein A Purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-ATG5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1:500-2000; IHC: 1:50-300
• Immunogen: Recombinant Protein of ATG5
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Hela cells using ATG5 Monoclonal Antibody at dilution of 1:10000.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Rat testis tissue using ATG5 Monoclonal Antibody at dilution of 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human ovarian carcinoma tissue using ATG5 Monoclonal Antibody at dilution of 1:200.)

Related Product Information for anti-ATG5 antibody

Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Product Categories/Family for anti-ATG5 antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 554790362
• NCBI GeneID: 9474
• NCBI Accession #: NP_001273035.1
• NCBI GenBank Nucleotide #: NM_001286106.1
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: 55kDa
• NCBI Official Full Name: autophagy protein 5 isoform a
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
• NCBI Protein Information: autophagy protein 5
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP

NCBI Description

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: autophagic vacuole; axoneme; cytoplasm; cytosol; ER-mitochondrion membrane contact site; membrane; phagocytic vesicle membrane; pre-autophagosomal structure membrane

Molecular Function: Atg8 ligase activity; protein binding

Biological Process: aggrephagy; antigen processing and presentation of endogenous antigen; apoptosis; autophagosome assembly; autophagy; autophagy of nucleus; blood vessel remodeling; C-terminal protein lipidation; cellular homeostasis; cellular response to nitrogen starvation; cellular response to nitrosative stress; heart contraction; innate immune response; macroautophagy; mitochondrion degradation; negative regulation of apoptosis; negative regulation of histone H4-K16 acetylation; negative regulation of phagocytosis; negative regulation of protein ubiquitination; negative regulation of reactive oxygen species metabolic process; negative thymic T cell selection; otolith development; post-translational protein modification; protein lipidation involved in autophagosome assembly; regulation of cilium assembly; regulation of cytokine secretion during immune response; regulation of release of sequestered calcium ion into cytosol; response to drug; response to fungus; vasodilation; ventricular cardiac muscle cell development

Disease: Spinocerebellar Ataxia, Autosomal Recessive 25