Western Blot (WB)
(Anti- Rad51C Picoband antibody, MBS178235, Western blottingAll lanes: Anti Rad51C (MBS178235) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
anti-Human Rad51C Polyclonal Antibody | anti-RAD51C antibody
Anti-Rad51C Antibody
Western Blot (WB)
(Anti- Rad51C Picoband antibody, MBS178235, Western blottingAll lanes: Anti Rad51C (MBS178235) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Western Blot (WB)
(Anti- Rad51C Picoband antibody, MBS178235, Western blottingAll lanes: Anti Rad51C (MBS178235) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )
Background: RAD51 homolog C (S. cerevisiae), also known as RAD51C, is a protein which in humans is encoded by the RAD51C gene. This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. Ana this gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in this gene are associated with Fanconi anemia-like syndrome. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
RAD51C: Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis. Defects in RAD51C are the cause of Fanconi anemia complementation group O (FANCO). It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. Defects in RAD51C are the cause of familial breast- ovarian cancer type 3 (BROVCA3). It is a condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Belongs to the RecA family. RAD51 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: condensed nuclear chromosome; cytoplasm; mitochondrion; nucleoplasm; nucleus; perinuclear region of cytoplasm; replication fork
Molecular Function: ATP binding; crossover junction endodeoxyribonuclease activity; DNA binding; DNA-dependent ATPase activity; double-stranded DNA binding; four-way junction DNA binding; protein binding; recombinase activity; single-stranded DNA binding
Biological Process: blood coagulation; DNA recombinase assembly; DNA recombination; DNA repair; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; female meiosis sister chromatid cohesion; male meiosis I; meiotic recombination; mitotic recombination; response to ionizing radiation; sister chromatid cohesion; spermatogenesis; strand displacement; strand invasion; telomere maintenance via recombination
Disease: Breast-ovarian Cancer, Familial, Susceptibility To, 3; Fanconi Anemia, Complementation Group O; Tracheoesophageal Fistula With Or Without Esophageal Atresia
Research Articles on RAD51C
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Product Notes
The RAD51C rad51c (Catalog #AAA178235) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rad51C Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rad51C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the RAD51C rad51c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad51C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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