Testing Data
(Peptide Competition Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1[pS282] antibody overnight at 4°C in a 1% milk- TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ETS1 [pS282] blocks the signal, verifying the specificity of the antibody. In addition, antibody blocking was not seen using phosphopeptides to the ETS1 pS251 or pS285 sites (not shown).)
Rabbit Ets 1 Polyclonal Antibody | anti-ETS1 antibody
Ets 1, phosphorylated (Ser282)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
Testing Data
(Peptide Competition Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1[pS282] antibody overnight at 4°C in a 1% milk- TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ETS1 [pS282] blocks the signal, verifying the specificity of the antibody. In addition, antibody blocking was not seen using phosphopeptides to the ETS1 pS251 or pS285 sites (not shown).)
Testing Data
(Peptide Competition Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1[pS282] antibody overnight at 4°C in a 1% milk- TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ETS1 [pS282] blocks the signal, verifying the specificity of the antibody. In addition, antibody blocking was not seen using phosphopeptides to the ETS1 pS251 or pS285 sites (not shown).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Uniprot Description
Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described.
Protein type: Oncoprotein; DNA-binding; Motility/polarity/chemotaxis; Transcription factor
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleus
Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; hypothalamus development; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; positive regulation of cell motility; female pregnancy; angiogenesis involved in wound healing; regulation of angiogenesis; response to estradiol stimulus; negative regulation of cell cycle; response to antibiotic; regulation of apoptosis; negative regulation of cell proliferation; positive regulation of angiogenesis; pituitary gland development; response to mechanical stimulus; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; immune response
Research Articles on ETS1
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Product Notes
The ETS1 ets1 (Catalog #AAA631088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ets 1, phosphorylated (Ser282) reacts with Frog, Human, Mouse, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Ets 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the ETS1 ets1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ets 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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