Rabbit anti-Human CAMKIV Polyclonal Antibody | anti-CAMK4 antibody

Anti-CAMKIV Antibody, Rabbit Polyclonal

Cat. Num. AAA8100644

• Gene Names: CAMK4; IV; caMK; CaMK IV; CaMK-GR
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CAMKIV; Polyclonal Antibody; Anti-CAMKIV Antibody; Rabbit Polyclonal; CaM Kinase 4/CaMKIV Antibody; Rabbit PAb; Antigen Affinity Purified; calcium/calmodulin-dependent protein kinase IV; Anti-caMK Antibody; Anti-CaMK-GR Antibody; Anti-CaMKIV Antibody; Anti-IV Antibody; anti-CAMK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CaM Kinase 4/CaMKIV
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CAMK4 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human CaM Kinase 4/CaMKIV
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CaM Kinase 4/CaMKIV, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CAMK4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa Observed band size:56 kDa)

Immunoprecipitation (IP)

(CAMK4 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CAMK4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAMK4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size :57 kDa)

Related Product Information for anti-CAMK4 antibody

Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca2+/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Ca2+ and then apt to combine with the target protein. Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKI¢ and CaMKIV have EF-hand structure.

Product Categories/Family for anti-CAMK4 antibody

EGFR Signaling Pathway

References

Feliciano DM, et al. (2009) Repression of Ca2+/calmodulin-dependent protein kinase IV signaling accelerates retinoic acid-induced differentiation of human neuroblastoma cells. J Biol Chem. 284 (39): 26466-81.Zhao X, et al. (2001). The modular nature of histone deacetylase HDAC4 confers phosphorylation-dependent intracellular trafficking. J Biol Chem. 276 (37): 35042-8.Racioppi L, et al. (2008) Calcium/calmodulin-dependent kinase IV in immune and inflammatory responses: novel routes for an ancient traveller.

NCBI and Uniprot Product Information

• NCBI GI #: 4502557
• NCBI GeneID: 814
• NCBI Accession #: NP_001735.1
• NCBI GenBank Nucleotide #: NM_001744.4
• UniProt Accession #: Q16566; D3DSZ7
• Molecular Weight: 51,926 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type IV
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase IV
• NCBI Official Symbol: CAMK4
• NCBI Official Synonym Symbols: IV; caMK; CaMK IV; CaMK-GR
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type IV; CAM kinase IV; caM kinase-GR; CAM kinase- GR; brain Ca++-calmodulin-dependent protein kinase type IV; brain Ca(2+)-calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinas
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type IV
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK4
• UniProt Synonym Gene Names: CAMK; CAMK-GR; CAMKIV; CaMK IV
• UniProt Entry Name: KCC4_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; regulation of T cell differentiation in the thymus; nucleocytoplasmic transport; myeloid dendritic cell differentiation; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production