Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. brain section using R Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Rabbit anti-Rat R Cdk4 Polyclonal Antibody | anti-Cdk4 antibody
R Cdk4 Antibody (C-term)
IF~~1:25
WB~~1:1000
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. brain section using R Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. brain section using R Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat small intestine section using Rat Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat small intestine section using Rat Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cells stained with (Rat) Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cells stained with (Rat) Cdk4 Antibody (C-term). MBS9210028 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(Rat Cdk4 Antibody (C-term) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow).)
Western Blot (WB)
(Rat Cdk4 Antibody (C-term) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
positive regulator of the cell cycle; plays a role in cell cycle progression [RGD, Feb 2006]
Uniprot Description
CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.
Protein type: Protein kinase, CMGC; EC 2.7.11.22; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily
Cellular Component: transcription factor complex; nuclear membrane; tight junction; perinuclear region of cytoplasm; nucleolus; cyclin-dependent protein kinase holoenzyme complex; nucleus; chromatin; cytosol
Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; protein complex binding; kinase activity; ATP binding; protein kinase activity
Biological Process: lens development in camera-type eye; circadian rhythm; response to drug; organ regeneration; positive regulation of cell size; positive regulation of translation; positive regulation of apoptosis; regulation of cell cycle; response to toxin; response to testosterone stimulus; signal transduction; protein amino acid phosphorylation; regulation of cell proliferation; response to organic substance; positive regulation of fibroblast proliferation; response to hyperoxia; cell division; regulation of gene expression; response to lead ion; positive regulation of cell proliferation; G1/S transition of mitotic cell cycle
Research Articles on Cdk4
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Product Notes
The Cdk4 cdk4 (Catalog #AAA9210028) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 272-303. The R Cdk4 Antibody (C-term) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's R Cdk4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). IHC~~1:25 IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the Cdk4 cdk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "R Cdk4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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