Human Radical S-adenosyl methionine domain-containing protein 2 ELISA Kit | RSAD2 elisa kit

Human Radical S-adenosyl methionine domain-containing protein 2 ELISA Kit

Cat. Num. AAA762507

• Gene Names: RSAD2; cig5; vig1; cig33; 2510004L01Rik
• Reactivity: Human
• Synonyms: Radical S-adenosyl methionine domain-containing protein 2; Human Radical S-adenosyl methionine domain-containing protein 2 ELISA Kit; RSAD2/Radical S-adenosyl methionine domain-containing protein 2/Virus inhibitory protein; endoplasmic reticulum-associated; interferon-inducible/Cytomegalovirus-induced gene 5 protein/Viperin; RSAD2 elisa kit

• Reactivity: Human
• Sequence Length: 361

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RSAD2 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RSAD2. During the reaction,Human RSAD2 in the sample or standard competes with a fixed amount of biotin-labeled Human RSAD2 for sites on a pre-coated Monoclonal antibody specific to Human RSAD2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human RSAD2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19923667
• NCBI GeneID: 91543
• NCBI Accession #: NP_542388.2
• NCBI GenBank Nucleotide #: NM_080657.4
• Molecular Weight: 42,170 Da
• NCBI Official Full Name: radical S-adenosyl methionine domain-containing protein 2
• NCBI Official Synonym Full Names: radical S-adenosyl methionine domain containing 2
• NCBI Official Symbol: RSAD2
• NCBI Official Synonym Symbols: cig5; vig1; cig33; 2510004L01Rik
• NCBI Protein Information: radical S-adenosyl methionine domain-containing protein 2
• UniProt Protein Name: Radical S-adenosyl methionine domain-containing protein 2
• Protein Family: Radical S-adenosyl methionine domain-containing protein
• UniProt Gene Name: RSAD2
• UniProt Entry Name: RSAD2_HUMAN

Uniprot Description

RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; lipid particle; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: 4 iron, 4 sulfur cluster binding; catalytic activity; metal ion binding; protein binding; protein self-association

Biological Process: CD4-positive, alpha beta T cell differentiation; cytokine and chemokine mediated signaling pathway; defense response to virus; metabolic process; negative regulation of protein secretion; negative regulation of viral genome replication; positive regulation of toll-like receptor 7 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; response to virus; viral reproduction