Human CD48 ELISA Kit | CD48 elisa kit

Human CD48 PicoKine ELISA Kit

Cat. Num. AAA178564

• Gene Names: CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• Reactivity: Human
• Synonyms: CD48; Human CD48 PicoKine ELISA Kit; Antigen CD48; BCM 1 surface antigen; Bcm-1; BCM1 surface antigen; BCM1; BLAST1; BLAST; CD48 antigen; CD48 molecule; CD48 protein; Leucocyte antigen MEM 102; Leukocyte antigen MEM-102; MEM102; SLAMF2; TCT.1; P09326; CD48 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD48
• Sequence Length: 243

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human CD48 PicoKine ELISA Kit standard curve )

Related Product Information for CD48 elisa kit

Principle of the Assay: Human CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD48 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q27-S220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD48 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 21361571
• NCBI GeneID: 962
• NCBI Accession #: NP_001769.2
• NCBI GenBank Nucleotide #: NM_001778.3
• UniProt Accession #: P09326; Q5U055; Q8MGR0
• Molecular Weight: 19,429 Da
• NCBI Official Full Name: CD48 antigen isoform 1
• NCBI Official Synonym Full Names: CD48 molecule
• NCBI Official Symbol: CD48
• NCBI Official Synonym Symbols: BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• NCBI Protein Information: CD48 antigen
• UniProt Protein Name: CD48 antigen
• Protein Family: CD48 antigen
• UniProt Gene Name: CD48
• UniProt Synonym Gene Names: BCM1; BLAST1; SLAMF2
• UniProt Entry Name: CD48_HUMAN

NCBI Description

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CD48: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q21.3-q22

Cellular Component: integral to plasma membrane; lipid raft; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: defense response; leukocyte migration