Rabbit STEAP4 Polyclonal Antibody | anti-STEAP4 antibody

Rabbit Polyclonal (IgG) to Human STEAP4

Cat. Num. AAA241321

• Gene Names: STEAP4; TIARP; STAMP2; TNFAIP9
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: STEAP4; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human STEAP4; Anti-STEAP4 Antibody (Internal) IHC-plus; STEAP family member 4; Metalloreductase STEAP4; STAMP2; TIARP; TNFAIP9; Human STEAP4; anti-STEAP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: a 14 amino acid peptide from near the center of human STEAP4
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 459

• Applicable Applications for anti-STEAP4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human STEAP4.
• Immunogen Type: Synthetic peptide
• Immunogen: STEAP4 antibody was raised against synthetic peptide from human STEAP4.
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-STEAP4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of STEAP4 in rat liver tissue lysate with STEAP4 antibody at (A) 0.5 and (B) 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 100815815
• NCBI GeneID: 79689
• NCBI Accession #: NP_078912.2
• NCBI GenBank Nucleotide #: NM_024636.3
• UniProt Accession #: Q687X5; Q658Q9; Q687X4; Q8WWB0; Q9H5R1
• Molecular Weight: 31,310 Da
• NCBI Official Full Name: metalloreductase STEAP4 isoform 1
• NCBI Official Synonym Full Names: STEAP family member 4
• NCBI Official Symbol: STEAP4
• NCBI Official Synonym Symbols: TIARP; STAMP2; TNFAIP9
• NCBI Protein Information: metalloreductase STEAP4; six transmembrane prostate protein 2; sixTransMembrane protein of prostate 2; tumor necrosis factor, alpha-induced protein 9; six-transmembrane epithelial antigen of prostate 4; tumor necrosis-alpha-induced adipose-related protein
• UniProt Protein Name: Metalloreductase STEAP4
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP4
• UniProt Synonym Gene Names: STAMP2; TNFAIP9
• UniProt Entry Name: STEA4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the STEAP (six transmembrane epithelial antigen of prostate) family, and resides in the golgi apparatus. It functions as a metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+), using NAD(+) as acceptor. Studies in mice and human suggest that this gene maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

STEAP4: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses. Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation. Belongs to the STEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; EC 1.16.1.-

Chromosomal Location of Human Ortholog: 7q21.12

Cellular Component: Golgi membrane; integral to membrane; plasma membrane; endosome

Molecular Function: metal ion binding; cupric reductase activity

Biological Process: fat cell differentiation; iron ion homeostasis; copper ion import