Rabbit anti-Human, Mouse PTPN22 Polyclonal Antibody | anti-PTPN22 antibody

PTPN22 Polyclonal Antibody

Cat. Num. AAA127647

• Gene Names: PTPN22; LYP; PEP; LYP1; LYP2; PTPN8
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: PTPN22; Polyclonal Antibody; PTPN22 Polyclonal Antibody; LYP; LYP1; LYP2; PEP; PTPN8; anti-PTPN22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 807

• Applicable Applications for anti-PTPN22 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human PTPN22
• Calculated Molecular Weight: 92kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PTPN22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Related Product Information for anti-PTPN22 antibody

PTPN22 (Lyp/PEP) is a cytoplasmic phosphatase expressed by hematopoietic cells (1,2). PTPN22 associates with the tyrosine kinase Csk to inhibit T cell receptor signaling through inactivation of Src kinases (3,4). Csk phosphorylates Src kinases on an inhibitory tyrosine, while PTPN22 dephosphorylates an activating site (4). PTPN22(-/-) mice have higher levels of activated Lck than wild-type, resulting in greater T cell expansion and increased serum antibody levels (5). Research studies have shown that a single-nucleotide polymorphism, 1858T of the PTPN22 gene which encodes the amino acid substitution R620W, confers increased risk for multiple autoimmune diseases including type I diabetes, rheumatoid arthritis, systemic lupus erythematosus, and Graves disease (6-9). Interestingly, although the R620W substitution disrupts the interaction between Csk and PTPN22, it is actually a gain-of-function mutation resulting in increased phosphatase activity (6,10,11). Recent evidence suggests that the autoimmune phenotype associated with the R620W variant is the result of increased calpain-mediated degradation and decreased protein levels of PTPN22 (12).

Product Categories/Family for anti-PTPN22 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 20139861
• NCBI GeneID: 26191
• UniProt Accession #: Q9Y2R2; O95063; O95064; Q6IPX8; Q8WVM1; A0N0K6; B1ALC8; D4NZ71; E9PLD8; E9PPI1
• Molecular Weight: 807
• NCBI Official Full Name: Tyrosine-protein phosphatase non-receptor type 22
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• NCBI Official Symbol: PTPN22
• NCBI Official Synonym Symbols: LYP; PEP; LYP1; LYP2; PTPN8
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 22; lymphoid phosphatase; PEST-domain phosphatase; lymphoid-specific protein tyrosine phosphatase; protein tyrosine phosphatase, non-receptor type 8; hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 22
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN22
• UniProt Synonym Gene Names: PTPN8; LyP; PEP
• UniProt Entry Name: PTN22_HUMAN

NCBI Description

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]

Uniprot Description

PTPN22: Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T-cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Interacts with CSK. Interacts with LPXN. Predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells. Down-regulated by phosphorylation. Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity; SH3 domain binding; kinase binding

Biological Process: regulation of B cell receptor signaling pathway; regulation of natural killer cell proliferation; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation; negative regulation of T cell activation; regulation of innate immune response; T cell differentiation

Disease: Diabetes Mellitus, Insulin-dependent; Systemic Lupus Erythematosus; Rheumatoid Arthritis