Rabbit PTPN22 Antibody | anti-PTPN22 antibody

PTPN22 Antibody

Cat. Num. AAA8501088

• Gene Names: PTPN22; LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: PTPN22; PTPN22 Antibody; LYP; LYP1; LYP2; PEP; PTPN8; anti-PTPN22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 779

• Applicable Applications for anti-PTPN22 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human PTPN22
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of Jurkatcell lines, using PTPN22 antibody.)

Related Product Information for anti-PTPN22 antibody

PTPN22 (Lyp/PEP) is a cytoplasmic phosphatase expressed by hematopoietic cells (1, 2). PTPN22 associates with the tyrosine kinase Csk to inhibit T cell receptor signaling through inactivation of Src kinases (3, 4). Csk phosphorylates Src kinases on an inhibitory tyrosine, while PTPN22 dephosphorylates an activating site (4). PTPN22(-/-) mice have higher levels of activated Lck than wild-type, resulting in greater T cell expansion and increased serum antibody levels (5). Research studies have shown that a single-nucleotide polymorphism, 1858T of the PTPN22 gene which encodes the amino acid substitution R620W, confers increased risk for multiple autoimmune diseases including type I diabetes, rheumatoid arthritis, systemic lupus erythematosus, and Graves disease (6-9). Interestingly, although the R620W substitution disrupts the interaction between Csk and PTPN22, it is actually a gain-of-function mutation resulting in increased phosphatase activity (6, 10, 11). Recent evidence suggests that the autoimmune phenotype associated with the R620W variant is the result of increased calpain-mediated degradation and decreased protein levels of PTPN22 (12).

Product Categories/Family for anti-PTPN22 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Cohen, S. et al. (1999) Blood 93, 2013-24. 2. Matthews, R.J. et al. (1992) Mol Cell Biol 12, 2396-405. 3. Cloutier, J.F. and Veillette, A. (1996) EMBO J 15, 4909-18. 4. Cloutier, J.F. and Veillette, A. (1999) J Exp Med 189, 111-21. 5. Hasegawa, K. et al. (2004) Science 303, 685-9. 6. Bottini, N. et al. (2004) Nat Genet 36, 337-8. 7. Begovich, A.B. et al. (2004) Am J Hum Genet 75, 330-7. 8. Kyogoku, C. et al. (2004) Am J Hum Genet 75, 504-7. 9. Velaga, M.R. et al. (2004) J Clin Endocrinol Metab 89, 5862-5. 10. Vang, T. et al. (2005) Nat Genet 37, 1317-9. 11. Rieck, M. et al. (2007) J Immunol 179, 4704-10. 12. Zhang, J. et al. (2011) Nat Genet 43, 902-7.

NCBI and Uniprot Product Information

• NCBI GI #: 301171669
• NCBI GeneID: 26191
• NCBI Accession #: NP_001180360.1
• NCBI GenBank Nucleotide #: NM_001193431.2
• UniProt Accession #: Q9Y2R2; O95063; O95064; Q6IPX8; Q8WVM1; A0N0K6; B1ALC8; D4NZ71; E9PLD8; E9PPI1
• Molecular Weight: 92kDa
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 22 isoform 3
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 22
• NCBI Official Symbol: PTPN22
• NCBI Official Synonym Symbols: LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 22
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 22
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN22
• UniProt Synonym Gene Names: PTPN8; LyP; PEP

NCBI Description

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]

Uniprot Description

Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules (PubMed:16461343, PubMed:18056643). Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue (PubMed:21719704). Dephosphorylates ZAP70 at its activating 'Tyr-493' residue (PubMed:16461343). Dephosphorylates the immune system activator SKAP2 (PubMed:21719704). Positively regulates toll-like receptor (TLR)-induced type 1 interferon production (PubMed:23871208). Promotes host antiviral responses mediated by type 1 interferon (). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy (PubMed:23991106).