Western Blot (WB)
(Western blot analysis of TM yeast Pst1 protein (50 ng) with Pst1 antibody at 1 μg/mL.)
Rabbit anti-Yeast Pst1 Polyclonal Antibody | anti-PST1 antibody
Pst1 Antibody
Gene Names
PST1; HPF2
Reactivity
Yeast
Applications
ELISA, Western Blot
Purity
Pst1 Antibody is affinity chromatography purified via peptide column.
Synonyms
Pst1; Polyclonal Antibody; Pst1 Antibody; HPF2; YDR055W; D4214; YD9609.09; Cell wall mannoprotein PST1; Haze protective factor 2; Protoplast secreted protein 1; anti-PST1 antibody
Host
Rabbit
Reactivity
Yeast
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Pst1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
444
Applicable Applications for anti-PST1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 mug/mL.
Conjugate
Unconjugated
Immunogen
Pst1 antibody was raised against a synthetic peptide from the amino terminus of yeast Pst1.
Buffer
Pst1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Pst1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of TM yeast Pst1 protein (50 ng) with Pst1 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of TM yeast Pst1 protein (50 ng) with Pst1 antibody at 1 μg/mL.)
Related Product Information for anti-PST1 antibody
Pst1 Antibody: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δoch1 Δmnn1 Δmnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49 kDa
NCBI Official Full Name
Cell wall mannoprotein PST1
NCBI Official Symbol
PST1
NCBI Official Synonym Symbols
HPF2
NCBI Protein Information
Pst1p
UniProt Protein Name
Cell wall mannoprotein PST1
Protein Family
UniProt Gene Name
PST1
UniProt Synonym Gene Names
HPF2
UniProt Entry Name
PST1_YEAST
Uniprot Description
Has a partially redundant function to ECM33 in cell wall integrity. May be involved in a repair mechanism activated in response to cell wall damage.
Research Articles on PST1
Similar Products
Product Notes
The PST1 pst1 (Catalog #AAA150678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pst1 Antibody reacts with Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's Pst1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 mug/mL. Researchers should empirically determine the suitability of the PST1 pst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pst1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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