Human CD93/C1qR ELISA Kit | CD93/C1qR elisa kit

Human CD93/C1qR PicoKine Fast ELISA Kit

Cat. Num. AAA1752770

• Gene Names: CD93; C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1
• Reactivity: Human
• Synonyms: CD93/C1qR; Human CD93/C1qR PicoKine Fast ELISA Kit; human CD93; Complement component C1q receptor; C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; Complement component 1 q subcomponent receptor 1; Matrix-remodeling-associated protein 4; CD93; C1QR1; MXRA4; CD93/C1qR elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD93
• Sequence Length: 652

• Samples: Cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD93/C1qR elisa kit

Principle of the Assay: The Picokine™ Human CD93 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD93 with a 96-well strip plate that is pre-coated with antibody specific for CD93. The detection antibody is a biotinylated antibody specific for CD93. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CD93 with immunogen: Expression system for standard: NSO; Immunogen sequence: A24-K580. The kit is analytically validated with ready to use reagents. To measure Human CD93, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD93 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD93 in the sample.!!Background/Introduction: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 88758613
• NCBI GeneID: 22918
• NCBI Accession #: NP_036204.2
• NCBI GenBank Nucleotide #: NP_036204.2
• UniProt Accession #: Q9NPY3
• NCBI Official Full Name: complement component C1q receptor
• NCBI Official Synonym Full Names: CD93 molecule
• NCBI Official Symbol: CD93
• NCBI Official Synonym Symbols: C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1
• NCBI Protein Information: complement component C1q receptor
• UniProt Protein Name: Complement component C1q receptor
• UniProt Gene Name: CD93
• UniProt Synonym Gene Names: C1QR1; MXRA4; C1qR; C1qR(p); C1qRp
• UniProt Entry Name: C1QR1_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. [provided by RefSeq, Jul 2008]