SDS-PAGE
(SDS PAGE analysis of Pst1 protein. Purified Pst1 protein (0.5 μg) was separated by 10% SDS PAGE gel, and stained with silver.)
TM Yeast Pst1 Recombinant Protein | PST1 recombinant protein
TM Yeast Pst1 Recombinant Protein
Gene Names
PST1; HPF2
Applications
ELISA, Western Blot
Purity
~95%
Synonyms
TM Yeast Pst1; TM Yeast Pst1 Recombinant Protein; HPF2; YDR055W; D4214; YD9609.09; Cell wall mannoprotein PST1; Haze protective factor 2; Protoplast secreted protein 1; PST1 recombinant protein
Purity/Purification
~95%
Form/Format
Liquid
Sequence Length
444
Applicable Applications for PST1 recombinant protein
ELISA (EIA), Western Blot (WB), MS
Application Notes
This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies. For research use only.
Source
S. cerevisiae TM strain (Deltaoch1 Deltamnn1 Deltamnn4)
Buffer
Lot specific, TBS or PBS
Preparation and Storage
Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
SDS-PAGE
(SDS PAGE analysis of Pst1 protein. Purified Pst1 protein (0.5 μg) was separated by 10% SDS PAGE gel, and stained with silver.)
SDS-PAGE
(SDS PAGE analysis of Pst1 protein. Purified Pst1 protein (0.5 μg) was separated by 10% SDS PAGE gel, and stained with silver.)
Related Product Information for PST1 recombinant protein
Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (deltaoch1 deltamnn1 deltamnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
100 kDa on SDS-PAGE
NCBI Official Full Name
Cell wall mannoprotein PST1
NCBI Official Symbol
PST1
NCBI Official Synonym Symbols
HPF2
NCBI Protein Information
Pst1p
UniProt Protein Name
Cell wall mannoprotein PST1
Protein Family
UniProt Gene Name
PST1
UniProt Synonym Gene Names
HPF2
UniProt Entry Name
PST1_YEAST
Uniprot Description
Has a partially redundant function to ECM33 in cell wall integrity. May be involved in a repair mechanism activated in response to cell wall damage.
Research Articles on PST1
Similar Products
Product Notes
The PST1 pst1 (Catalog #AAA153313) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TM Yeast Pst1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), MS. This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies. For research use only. Researchers should empirically determine the suitability of the PST1 pst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TM Yeast Pst1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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