Canine Myocyte-specific enhancer factor 2C (MEF2C) ELISA Kit | MEF2C elisa kit

Canine Myocyte-specific enhancer factor 2C (MEF2C) ELISA Kit

Cat. Num. AAA7238567

• Gene Names: MEF2C; DEL5q14.3; C5DELq14.3
• Reactivity: Canine
• Synonyms: Myocyte-specific enhancer factor 2C (MEF2C); Canine Myocyte-specific enhancer factor 2C (MEF2C) ELISA Kit; MEF2C elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of MEF2C. No significant cross-reactivity or interference between MEF2C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MEF2C and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MEF2C elisa kit

Intended Uses: This MEF2C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MEF2C. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: MEF2C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MEF2C antibody and an MEF2C-HRP conjugate. The assay sample and buffer are incubated together with MEF2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MEF2C concentration since MEF2C from samples and MEF2C-HRP conjugate compete for the anti-MEF2C antibody binding site. Since the number of sites is limited, as more sites are occupied by MEF2C from the sample, fewer sites are left to bind MEF2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MEF2C concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MEF2C elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 19923215
• NCBI GeneID: 4208
• NCBI Accession #: NP_002388.2
• NCBI GenBank Nucleotide #: NM_002397.4
• Molecular Weight: 50,242 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2C isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2C
• NCBI Official Symbol: MEF2C
• NCBI Official Synonym Symbols: DEL5q14.3; C5DELq14.3
• NCBI Protein Information: myocyte-specific enhancer factor 2C; MADS box transcription enhancer factor 2, polypeptide C
• UniProt Protein Name: Myocyte-specific enhancer factor 2C
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2C
• UniProt Entry Name: MEF2C_HUMAN

NCBI Description

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Uniprot Description

MEF2C: transcription factor of the MADS family which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in myogenesis, neurogenesis and in the development of cortical architecture. Three splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: nucleoplasm; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; transcription activator binding; DNA binding; AT DNA binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; muscle development; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; heart development; regulation of neurotransmitter secretion; stress-activated MAPK cascade; regulation of synaptic plasticity; toll-like receptor 3 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; positive regulation of MAP kinase activity; B cell receptor signaling pathway; regulation of neuron apoptosis; positive regulation of B cell proliferation; negative regulation of ossification; chondrocyte differentiation; negative regulation of neuron apoptosis; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; endochondral ossification; nervous system development; skeletal muscle development; monocyte differentiation; response to virus; myotube differentiation; positive regulation of skeletal muscle development; muscle cell fate determination; toll-like receptor 2 signaling pathway; muscle cell differentiation; regulation of synaptogenesis; neuron development; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; B cell proliferation; transcription from RNA polymerase II promoter; regulation of synaptic transmission, glutamatergic; apoptosis; ventricular cardiac muscle cell differentiation; neuron migration; regulation of synaptic activity; negative regulation of transcription from RNA polymerase II promoter; palate development; toll-like receptor 10 signaling pathway; learning and/or memory; regulation of transcription, DNA-dependent; B cell homeostasis; regulation of megakaryocyte differentiation; melanocyte differentiation; germinal center formation; heart looping; neural crest cell differentiation; negative regulation of epithelial cell proliferation; blood vessel development; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; embryonic viscerocranium morphogenesis; positive regulation of bone mineralization; regulation of germinal center formation; humoral immune response; positive regulation of myoblast differentiation; osteoblast differentiation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of osteoblast differentiation; neuron morphogenesis during differentiation; toll-like receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; blood vessel remodeling; platelet formation; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation

Disease: Mental Retardation, Autosomal Dominant 20