Western Blot (WB)
(DAPP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DAPP1 antibody detected the DAPP1 protein (arrow).)
Rabbit anti-Human DAPP1 Polyclonal Antibody | anti-DAPP1 antibody
DAPP1, CT (DAPP1, BAM32, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, B lymphocyte adapter protein Bam32, B Cell adapter molecule of 32kD) (Biotin)
Gene Names
DAPP1; BAM32
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DAPP1; Polyclonal Antibody; CT (DAPP1; BAM32; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; B lymphocyte adapter protein Bam32; B Cell adapter molecule of 32kD) (Biotin); B-cell adapter molecule of 32kD; anti-DAPP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-DAPP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
DAPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-275 amino acids from the C-terminal region of human DAPP1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(DAPP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DAPP1 antibody detected the DAPP1 protein (arrow).)
Western Blot (WB)
(DAPP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DAPP1 antibody detected the DAPP1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of DAPP1 (arrow) using rabbit polyclonal DAPP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DAPP1 gene.)
Western Blot (WB)
(Western blot analysis of DAPP1 (arrow) using rabbit polyclonal DAPP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DAPP1 gene.)
Related Product Information for anti-DAPP1 antibody
DAPP1 may act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Product Categories/Family for anti-DAPP1 antibody
References
Manna, D., et al. J. Biol. Chem. 282(44):32093-32105(2007). Niiro, H., et al. J. Immunol. 173(9):5601-5609(2004). Allam, A., et al. J. Biol. Chem. 279(38):39775-39782(2004). Han, A., et al. Immunity 19(4):621-632(2003). Marshall, A.J., et al. Mol. Cell. Biol. 22(15):5479-5491(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,194 Da
NCBI Official Full Name
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
NCBI Official Synonym Full Names
dual adaptor of phosphotyrosine and 3-phosphoinositides
NCBI Official Symbol
DAPP1
NCBI Official Synonym Symbols
BAM32
NCBI Protein Information
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; hDAPP1; B-cell adapter molecule of 32 kDa; b lymphocyte adapter protein Bam32
UniProt Protein Name
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
UniProt Gene Name
DAPP1
UniProt Synonym Gene Names
BAM32; hDAPP1
UniProt Entry Name
DAPP1_HUMAN
Uniprot Description
DAPP1: an adaptor protein. Regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q25-q27
Cellular Component: plasma membrane; cytosol
Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; phospholipid binding; phosphatidylinositol-3,4-bisphosphate binding
Biological Process: signal transduction; protein amino acid dephosphorylation
Research Articles on DAPP1
Similar Products
Product Notes
The DAPP1 dapp1 (Catalog #AAA6290828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAPP1, CT (DAPP1, BAM32, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, B lymphocyte adapter protein Bam32, B Cell adapter molecule of 32kD) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAPP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DAPP1 dapp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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