Rabbit PSMD10 Polyclonal Antibody | anti-PSMD10 antibody

PSMD10 Polyclonal Antibody

Cat. Num. AAA127030

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: PSMD10; Polyclonal Antibody; PSMD10 Polyclonal Antibody; p28; p28(GANK); dJ889N15.2; anti-PSMD10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 226

• Applicable Applications for anti-PSMD10 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human PSMD10
• Calculated Molecular Weight: 24kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of SW620 cells, using PSMD10 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using PSMD10 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using PSMD10 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse spleen using PSMD10 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-PSMD10 antibody

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Product Categories/Family for anti-PSMD10 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 20532206
• NCBI GeneID: 5716
• UniProt Accession #: O75832; Q5U0B2; Q8IZK9
• Molecular Weight: 226
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 10
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10; gankyrin; ankyrin repeat protein; 26S proteasome regulatory subunit p28; hepatocellular carcinoma-associated protein p28-II
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD10
• UniProt Entry Name: PSD10_HUMAN

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Uniprot Description

PSMD10: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: proteasome complex; nucleoplasm; intermediate filament cytoskeleton; proteasome regulatory particle; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; protein polyubiquitination; viral reproduction; apoptosis; negative regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; antigen processing and presentation of peptide antigen via MHC class I; cytoplasmic sequestering of NF-kappaB; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis