ESRP1/RBM35A Recombinant Protein | ESRP1 recombinant protein

ESRP1/RBM35A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121512

• Gene Names: ESRP1; RBM35A; RMB35A
• Purity: >65% as determined by SDS-PAGE
• Synonyms: ESRP1/RBM35A; ESRP1/RBM35A Protein; Human; Recombinant (His Tag); Human ESRP1 Protein (His Tag); RBM35A Protein; RMB35A Protein; epithelial splicing regulatory protein 1; ESRP1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >65% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, pH7.5, 10% glycerol, 0.5mM TCEP. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile681

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ESRP1 (NP_060167.2)(Met1-Ile681) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ESRP1 recombinant protein

Background: Epithelial splicing regulatory protein 1 (ESRP1) is an epithelial cell-specific RNA binding protein that controls several key cellular processes, like alternative splicing and translation. Epithelial splicing regulatory protein 1 (ESRP1) has been characterized as an essential splicing factor required for the regulation of human pluripotency and differentiation. ESRP1 regulates CD44 alternative splicing during the EMT process which plays an important role in EOC carcinogenesis. In addition, ESRP1 is associated with disease prognosis in epithelial ovarian cancer (EOC). Epithelial splicing regulatory protein 1 (ESRP1) and 2 (ESRP2), epithelial cell-specific regulators of alternative splicing, are downregulated during the epithelial-mesenchymal transition (EMT). There is a critical role of the AS regulator epithelial splicing regulator protein 1 (ESRP1) for intestinal homeostasis and pathogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 56790297
• NCBI GeneID: 54845
• NCBI Accession #: NP_060167.2
• NCBI GenBank Nucleotide #: NM_017697.3
• UniProt Accession #: Q6NXG1; Q2M2B0; Q499G3; Q6PJ86; Q9NXL8; A6NHA8; A8MPX1; E9PB47
• Molecular Weight: 73,133 Da
• NCBI Official Full Name: epithelial splicing regulatory protein 1 isoform 1
• NCBI Official Synonym Full Names: epithelial splicing regulatory protein 1
• NCBI Official Symbol: ESRP1
• NCBI Official Synonym Symbols: RBM35A; RMB35A
• NCBI Protein Information: epithelial splicing regulatory protein 1; RNA-binding protein 35A; RNA binding motif protein 35A; RNA-binding motif protein 35A
• UniProt Protein Name: Epithelial splicing regulatory protein 1
• Protein Family: Epithelial splicing regulatory protein
• UniProt Gene Name: ESRP1
• UniProt Synonym Gene Names: RBM35A
• UniProt Entry Name: ESRP1_HUMAN

NCBI Description

ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009]

Uniprot Description

RBM35A: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: nucleus

Molecular Function: mRNA binding; nucleotide binding

Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing