Rabbit anti-Human PSMD10 Polyclonal Antibody | anti-PSMD10 antibody

PSMD10 (26S Proteasome Non-ATPase Regulatory Subunit 10, 26S Proteasome Regulatory Subunit p28, Gankyrin, p28(GANK)) (Biotin)

Cat. Num. AAA6391156

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMD10; Polyclonal Antibody; PSMD10 (26S Proteasome Non-ATPase Regulatory Subunit 10; 26S Proteasome Regulatory Subunit p28; Gankyrin; p28(GANK)) (Biotin); anti-PSMD10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMD10.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PSMD10 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PSMD10, aa1-226 (NP_002805.1).
• Immunogen Sequence: MEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PSMD10 expression in transfected 293T cell line by PSMD10 rabbit polyclonal antibody. Lane 1: PSMD10 transfected lysate (24.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PSMD10 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

Product Categories/Family for anti-PSMD10 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 4506217
• NCBI GeneID: 5716
• NCBI Accession #: NP_002805.1
• NCBI GenBank Nucleotide #: NM_002814
• UniProt Accession #: O75832; Q5U0B2; Q8IZK9
• Molecular Weight: 16,137 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 10 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; gankyrin; hepatocellular carcinoma-associated protein p28-II
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD10
• UniProt Entry Name: PSD10_HUMAN

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Uniprot Description

PSMD10: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: proteasome complex; nucleoplasm; intermediate filament cytoskeleton; proteasome regulatory particle; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; protein polyubiquitination; viral reproduction; apoptosis; negative regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; antigen processing and presentation of peptide antigen via MHC class I; cytoplasmic sequestering of NF-kappaB; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis