Mouse CDCP1 Monoclonal Antibody | anti-CDCP1 antibody

CDCP1 antibody

Cat. Num. AAA830203

• Gene Names: CDCP1; CD318; TRASK; SIMA135
• Applications: Western Blot
• Purity: CDCP1 antibody was purified by affinity chromatography.
• Synonyms: CDCP1; Monoclonal Antibody; CDCP1 antibody; Monoclonal CDCP1; Anti-CDCP1; CD318; Cluster of differentiation 318; Trask; Transmembrane associated with src kinases; CDCP 1; CDCP-1; anti-CDCP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F1
• Purity/Purification: CDCP1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 836

• Applicable Applications for anti-CDCP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Biological Significance: CDCP1 is a transmembrane protein containing three extracellular CUB domains. This protein isfound to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability ofcarcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in acancer cell line.
• Cross-Reactivity: Human
• Immunogen: CDCP1 antibody was raised in mouse using a full length recombinant protein of human CDCP1 (NP_073753) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot

(Western Blot analysis using CDCP1 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CDCP1 protein (Right) or empty vector (Left) detected with CDCP1 antibody)

Related Product Information for anti-CDCP1 antibody

Mouse monoclonal CDCP1 antibody

Product Categories/Family for anti-CDCP1 antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 30410805
• NCBI GeneID: 64866
• NCBI Accession #: NP_073753
• NCBI GenBank Nucleotide #: NM_022842.3
• UniProt Accession #: Q9H5V8; Q49UB4; Q6NT71; Q6U9Y2; Q8WU91; Q96QU7; Q9H676; Q9H8C2
• Molecular Weight: 37,818 Da
• NCBI Official Full Name: CUB domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: CDCP1
• NCBI Official Synonym Symbols: CD318; TRASK; SIMA135
• NCBI Protein Information: CUB domain-containing protein 1
• UniProt Protein Name: CUB domain-containing protein 1
• Protein Family: CUB domain-containing protein
• UniProt Gene Name: CDCP1
• UniProt Synonym Gene Names: TRASK; SIMA135
• UniProt Entry Name: CDCP1_HUMAN

NCBI Description

This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular region; plasma membrane; integral to membrane