Rabbit anti-Human PSMB1 Polyclonal Antibody | anti-PSMB1 antibody

PSMB1 Antibody

Cat. Num. AAA9407583

• Gene Names: PSMB1; HC5; PSC5; PMSB1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PSMB1; Polyclonal Antibody; PSMB1 Antibody; HC5; PSC5; PMSB1; anti-PSMB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PSMB1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 241

• Applicable Applications for anti-PSMB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PSMB1.
• Target Name: PSMB1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMB1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast cancer using PSMB1 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat testis using PSMB1 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using PSMB1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PSMB1 antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.

Product Categories/Family for anti-PSMB1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506193
• NCBI GeneID: 5689
• NCBI Accession #: NP_002784.1
• NCBI GenBank Nucleotide #: NM_002793.3
• UniProt Accession #: P20618; Q9BWA8; B5BU76
• Molecular Weight: 26,489 Da
• NCBI Official Full Name: proteasome subunit beta type-1
• NCBI Official Synonym Full Names: proteasome subunit beta 1
• NCBI Official Symbol: PSMB1
• NCBI Official Synonym Symbols: HC5; PSC5; PMSB1
• NCBI Protein Information: proteasome subunit beta type-1
• UniProt Protein Name: Proteasome subunit beta type-1
• Protein Family: Proteasome
• UniProt Gene Name: PSMB1
• UniProt Synonym Gene Names: PSC5
• UniProt Entry Name: PSB1_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMB1: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Protein type: Protease; Cell cycle regulation; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis