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Immunohistochemistry (IHC) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Ewing's Sarcoma using CD99 antibody.)

Mouse anti-Human CD99 Monoclonal Antibody | anti-CD99 antibody

Anti-CD99 Antibody [ABT-CD99]

Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human
Applications
Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
CD99; Monoclonal Antibody; Anti-CD99 Antibody [ABT-CD99]; Anti-CD99 [ABT-CD99]; Mouse monoclonal [ABT-CD99] antibody to CD99; CD99 antigen; T-cell surface glycoprotein E2; E2 antigen; Protein MIC2; 12E7; MIC2; MIC2X; MIC2Y; anti-CD99 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
ABT-CD99
Purity/Purification
Immunogen Affinity Purified
Form/Format
0.5% Bovine Serum Albumin (BSA), 0.1% Kathon CG/ICP, 10x Tris-Buffered Saline (TBS), Type 3 MilliQ H2O and 50% Glycerol.
Sequence Length
185
Applicable Applications for anti-CD99 antibody
Immunohistochemistry (IHC)
Application Notes
Dilution: 1:50-200
Immunogen
Synthetic Peptide
Localization
Membranous
IHC Recommended Control
Ewing's Sarcoma, Pancreas
Cell Marque/Ventana Control
Ewing's Sarcoma
Retrieval Buffer
TRIS-EDTA of pH 8.0
Antigen Retrieval
Heat-Induced Epitope Retrieval (HIER)
Preparation and Storage
Store at -20 degree C for 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Ewing's Sarcoma using CD99 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Ewing's Sarcoma using CD99 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Ewing's Sarcoma using CD99 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human Ewing's Sarcoma using CD99 antibody.)
Product Categories/Family for anti-CD99 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD99 antigen isoform a
NCBI Official Synonym Full Names
CD99 molecule (Xg blood group)
NCBI Official Symbol
CD99
NCBI Official Synonym Symbols
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
NCBI Protein Information
CD99 antigen
UniProt Protein Name
CD99 antigen
Protein Family
UniProt Gene Name
CD99
UniProt Synonym Gene Names
MIC2; MIC2X; MIC2Y
UniProt Entry Name
CD99_HUMAN

NCBI Description

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]

Uniprot Description

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion

Research Articles on CD99

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Product Notes

The CD99 cd99 (Catalog #AAA9510644) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD99 Antibody [ABT-CD99] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD99 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Dilution: 1:50-200. Researchers should empirically determine the suitability of the CD99 cd99 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD99, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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