Rabbit anti-Human Proteasome subunit beta type-1 Polyclonal Antibody | anti-PSMB1 antibody

Rabbit anti-human Proteasome subunit beta type-1 polyclonal Antibody

Cat. Num. AAA7004639

• Gene Names: PSMB1; HC5; PSC5; PMSB1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Proteasome subunit beta type-1; Polyclonal Antibody; Rabbit anti-human Proteasome subunit beta type-1 polyclonal Antibody; PSC5; PSMB1; Macropain subunit C5 Multicatalytic endopeptidase complex subunit C5 Proteasome component C5 Proteasome gamma chain; anti-PSMB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 241

• Applicable Applications for anti-PSMB1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilution: WB:1:500-2000
• Immunogen: Recombinant human Proteasome subunit beta type-1 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-67345
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: Proteasome subunit beta 1 antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 26.4 kDaObserved band size: 26.4 kDa)

Related Product Information for anti-PSMB1 antibody

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.Cleavage of peptide bonds with very broad specificity.The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with SERPINB2. Interacts with HIV-1 TAT protein.

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.

Product Categories/Family for anti-PSMB1 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4506193
• NCBI GeneID: 5689
• NCBI Accession #: NP_002784.1
• NCBI GenBank Nucleotide #: NM_002793.3
• UniProt Accession #: P20618; Q9BWA8; B5BU76
• Molecular Weight: 26,489 Da
• NCBI Official Full Name: proteasome subunit beta type-1
• NCBI Official Synonym Full Names: proteasome subunit beta 1
• NCBI Official Symbol: PSMB1
• NCBI Official Synonym Symbols: HC5; PSC5; PMSB1
• NCBI Protein Information: proteasome subunit beta type-1
• UniProt Protein Name: Proteasome subunit beta type-1
• Protein Family: Proteasome
• UniProt Gene Name: PSMB1
• UniProt Synonym Gene Names: PSC5
• UniProt Entry Name: PSB1_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMB1: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Protein type: EC 3.4.25.1; Protease; Cell cycle regulation; Proteasome complex

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex

Molecular Function: protein binding; threonine endopeptidase activity

Biological Process: activation of MAPKK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; apoptosis; axon guidance; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; gene expression; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; mitotic cell cycle; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; nerve growth factor receptor signaling pathway; polyamine metabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; programmed cell death; protein polyubiquitination; Ras protein signal transduction; regulation of amino acid metabolic process; regulation of apoptosis; regulation of mRNA stability; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction