Kininogen 1 Active Protein | KNG1 active protein

Kininogen 1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122768

• Gene Names: Kng1; Kng
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Kininogen 1; Kininogen 1 Protein; Mouse; Recombinant (His Tag); Mouse KNG1/BDK/kininogen-1 Protein (His Tag); Kng Protein; kininogen 1; KNG1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mM NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu21-Ser480

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse KNG1 isoform 3 (NP_001095882.1)(Glu 21-Ser 480) was expressed, with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is <5nM.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KNG1 active protein

Background: Kininogen-1, also known as high molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, KNG1, and BDK, is a secreted protein that contains three cystatin domains. Kininogen-1/KNG1 is a protein from the blood coagulation system as well as the kinin-kallikrein system. It is a protein that adsorbs to the surface of biomaterials that come in contact with blood. Kininogen-1/KNG1 circulates throughout the blood and quickly adsorbs to the material surfaces. Kininogen-1/KNG1 is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein. Kininogen-1/KNG1 is one of the kininogens, a class of proteins. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed. When the clinical data were combined, it turned out that all patients had a deficiency of the same protein. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) which is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

Product Categories/Family for KNG1 active protein

Neurotransmitter Associated Enzymes

References

Ohkubo I., et al.,(1984), Isolation of a human cDNA for alpha 2-thiol proteinase inhibitor and its identity with low molecular weight kininogen. Biochemistry 23:5691-5697.Takagaki Y., et al., (1985), Cloning and sequence analysis of cDNAs for human high molecular weight and low molecular weight prekininogens. Primary structures of two human prekininogens.J. Biol. Chem. 260:8601-8609.Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 156231023
• NCBI GeneID: 16644
• NCBI Accession #: NP_001095882.1
• NCBI GenBank Nucleotide #: NM_001102412.1
• Molecular Weight: 53,206 Da
• NCBI Official Full Name: kininogen-1 isoform 3
• NCBI Official Synonym Full Names: kininogen 1
• NCBI Official Symbol: Kng1
• NCBI Official Synonym Symbols: Kng
• NCBI Protein Information: kininogen-1
• Protein Family: Kininogen