Western Blot (WB)
(Western Blot analysis using MBS617526. Jurkat cells were either untreated or treated with 0.1uM calyculin A for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting.)
Rabbit Akt Substrate Polyclonal Antibody | anti-Akt1 antibody
Akt Substrate, Phosphorylated (Ser/Thr) (Rac PKa, PKBa)
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Dilution: ELISA-Peptide: 1:500
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:300 using citrate as an unmasking buffer.
Western Blot (WB)
(Western Blot analysis using MBS617526. Jurkat cells were either untreated or treated with 0.1uM calyculin A for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting.)
Western Blot (WB)
(Western Blot analysis using MBS617526. Jurkat cells were either untreated or treated with 0.1uM calyculin A for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting.)
Western Blot (WB)
(Western Blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using MBS617526)
Western Blot (WB)
(Western Blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using MBS617526)
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells, untreated or insulin-treated (100nM for 15 minutes prior to lysis), using MBS617526.)
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells, untreated or insulin-treated (100nM for 15 minutes prior to lysis), using MBS617526.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MBS617526)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MBS617526)
NCBI and Uniprot Product Information
Uniprot Description
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.
Protein type: Oncoprotein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; AGC group; AKT family
Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; phosphoinositide binding; ATP binding; protein kinase activity
Biological Process: circadian rhythm; oogenesis; positive regulation of cell size; chitin-based embryonic cuticle biosynthetic process; regulation of dendrite development; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; positive regulation of organ growth; positive regulation of cell growth; epithelial cell migration, open tracheal system; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; response to oxidative stress; lipid metabolic process; regulation of cell size; negative regulation of apoptosis
Research Articles on Akt1
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Product Notes
The Akt1 akt1 (Catalog #AAA617526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Akt Substrate can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: ELISA-Peptide: 1:500 Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:300 using citrate as an unmasking buffer. Researchers should empirically determine the suitability of the Akt1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt Substrate, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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