Mouse Tyrosine Monoclonal Antibody | anti-TH antibody

Tyrosine, phosphorylated (Tyr)

Cat. Num. AAA633528

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Bovine, Canine, Chicken, Goat, Human, Mouse, Rabbit, Rat, Sheep
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: Tyrosine; Monoclonal Antibody; phosphorylated (Tyr); Anti -Tyrosine; anti-TH antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Goat, Human, Mouse, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A41
• Specificity: Binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequencein ELISA. Binds to a larger number of phospho-Tyr-containing peptides than any other commercially available anti-phospho-Tyr monoclonal antibody. Does not crossreact with peptides containing phospho-Ser or phospho-Thr. Species Crossreactivity: All species
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-TH antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA, Immunofluorescence and Flow Cytometry.; Dilution: Western Blot: 1:2000; Immunoprecipitation: 1:100; Immunohistochemistry (Paraffin): 1:100; Immunofluoresence (frozen): 1:400; Immunofluorescence (IC): 1:400; ELISA: 1:1000; Flow Cytometry: 1:400
• Immunogen: Phospho-tyrosine-containing peptides (KLH coupled)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Tyrosine phosphorylation in rat hippocampal dentate gyrus ischemia. Control (lanes 1 and 2); 15 minute transient cerebral ischemia (lanes 3 and 4); reperfusion for 4 hours (lanes 5 and 6); and 24 hours (lanes 7 and 8). Western Blot analysis using MBS633528 (Provided by Dr. Bingren Hu, Queen’s Medical Center, Hawaii.))

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using MBS633528.)

Related Product Information for anti-TH antibody

Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. Phospho-tyrosine monoclonal antibodies provide exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 88900505
• NCBI GeneID: 7054
• NCBI Accession #: NP_954987.2
• NCBI GenBank Nucleotide #: NM_199293.2
• UniProt Accession #: P07101; Q15585; Q15588; Q15589; B7ZL70
• Molecular Weight: 58,600 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase isoform c
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• Protein Family: Tyrosine permease
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: EC 1.14.16.2; Mitochondrial; Vesicle; Oxidoreductase; Endoplasmic reticulum; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to pyrethroid; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; cerebral cortex development; response to activity; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to salt stress; regulation of heart contraction; response to estradiol stimulus; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; memory; synaptic vesicle amine transport; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive