Rabbit anti-Human Protein Kinase A, alpha/beta, Catalytic Subunit Polyclonal Antibody
Protein Kinase A, alpha/beta, Catalytic Subunit, phosphorylated (Thr197) (PKAa,b)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Protein Kinase A; alpha/beta; Catalytic Subunit; Polyclonal Antibody; phosphorylated (Thr197) (PKAa; b); Anti -Protein Kinase A; anti-Protein Kinase A; Catalytic Subunit antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PKA catalytic subunits a and b when phosphorylated at Thr197. Species sequence homology: mouse, rat, bovine, porcine and frog.This antibody exibited a preference for PKA catalytic subunit b in some cell lines tested.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
Applicable Applications for anti-Protein Kinase A, alpha/beta, Catalytic Subunit antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 0.1-0.75ug/ml. At 0.35ug/ml, the dilution provides 100ml working solution, which at 10ml/blot allows 10 blots to be performed.
Dilution: Western Blot: 0.1-0.75ug/ml. At 0.35ug/ml, the dilution provides 100ml working solution, which at 10ml/blot allows 10 blots to be performed.
Immunogen
Synthetic phosphopeptide derived from a region of human PKA that contains threonine 197.
Positive Control
Forskolin-treated NIH3T3 cells, or Y-1 mouse adrenal cortical cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Protein Kinase A, alpha/beta, Catalytic Subunit antibody
c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop. Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions.
Product Categories/Family for anti-Protein Kinase A, alpha/beta, Catalytic Subunit antibody
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Product Notes
The Protein Kinase A, alpha/beta, Catalytic Subunit (Catalog #AAA610853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Kinase A, alpha/beta, Catalytic Subunit, phosphorylated (Thr197) (PKAa,b) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase A, alpha/beta, Catalytic Subunit can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.1-0.75ug/ml. At 0.35ug/ml, the dilution provides 100ml working solution, which at 10ml/blot allows 10 blots to be performed. Researchers should empirically determine the suitability of the Protein Kinase A, alpha/beta, Catalytic Subunit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase A, alpha/beta, Catalytic Subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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