Chicken CCL14 ELISA Kit | CCL14 elisa kit

Chicken CCL14 (Chemokine C-C-Motif Ligand 14) ELISA Kit

Cat. Num. AAA2501117

• Gene Names: CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• Reactivity: Chicken
• Synonyms: CCL14; Chicken CCL14 (Chemokine C-C-Motif Ligand 14) ELISA Kit; CCL14 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken CCL14. No significant cross-reactivity or interference between Chicken CCL14 and analogues was observed.
• Sequence Length: 93

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50pg/mL
• Sensitivity: Min: 0.469pg/mL; Max: 50pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL14 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken CCL14 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CCL14. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CCL14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CCL14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CCL14. You can calculate the concentration of CCL14 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14589961
• NCBI GeneID: 6358
• NCBI Accession #: NP_116739.1
• NCBI GenBank Nucleotide #: NM_032963.3
• UniProt Accession #: Q16627; Q13954; E1P649; E1P650
• Molecular Weight: 12,297 Da
• NCBI Official Full Name: C-C motif chemokine 14 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 14
• NCBI Official Symbol: CCL14
• NCBI Official Synonym Symbols: CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• NCBI Protein Information: C-C motif chemokine 14; chemokine CC-1/CC-3; chemokine CC-3; hemofiltrate CC chemokine 1; new CC chemokine 2; small inducible cytokine subfamily A (Cys-Cys), member 14; small-inducible cytokine A14
• UniProt Protein Name: C-C motif chemokine 14
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL14
• UniProt Synonym Gene Names: NCC2; SCYA14; HCC-1/HCC-3
• UniProt Entry Name: CCL14_HUMAN

NCBI Description

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]

Uniprot Description

CCL14: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; positive regulation of cell proliferation; immune response