Typical Testing Data/Standard Curve (for reference only)
Mouse Epididymis Protein 4 ELISA Kit | HE-4 elisa kit
Mouse Epididymis Protein 4 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: HE4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HE4 antibody and an HE4-HRP conjugate. The assay sample and buffer are incubated together with HE4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HE4 concentration since HE4 from samples and HE4-HRP conjugate compete for the anti-HE4 antibody binding site. Since the number of sites is limited, as more sites are occupied by HE4 from the sample, fewer sites are left to bind HE4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HE4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]
Uniprot Description
WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: extracellular space
Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity
Biological Process: spermatogenesis; proteolysis
Research Articles on HE-4
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Product Notes
The Mouse HE-4 wfdc2 (Catalog #AAA752662) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA752662 ELISA Kit recognizes Mouse HE-4. It is sometimes possible for the material contained within the vial of "Epididymis Protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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