Western Blot (WB)
(PRKAR1B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAR1B antibody detected the PRKAR1B protein (arrow).)
Rabbit anti-Human PRKAR1B Polyclonal Antibody | anti-PRKAR1B antibody
PRKAR1B, NT (PRKAR1B, cAMP-dependent protein kinase type I-beta regulatory subunit)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(PRKAR1B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAR1B antibody detected the PRKAR1B protein (arrow).)
Western Blot (WB)
(PRKAR1B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAR1B antibody detected the PRKAR1B protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM, Jun 2009]
Uniprot Description
Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Ref.9
Subunit structure: The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane. Ref.9 Ref.10
Subcellular location: Cell membrane Ref.10.
Tissue specificity: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Post-translational modification: The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Sequence similarities: Belongs to the cAMP-dependent kinase regulatory chain family.Contains 2 cyclic nucleotide-binding domains.
Research Articles on PRKAR1B
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Product Notes
The PRKAR1B prkar1b (Catalog #AAA648017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKAR1B, NT (PRKAR1B, cAMP-dependent protein kinase type I-beta regulatory subunit) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKAR1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PRKAR1B prkar1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKAR1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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