Western Blot (WB)
(Western Blot analysis using MBS631158.)
Rabbit anti-Human PKR Polyclonal Antibody | anti-pkr antibody
PKR, phosphorylated (Thr451) (ds RNA-dependent Protein Kinase)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PKR; Polyclonal Antibody; phosphorylated (Thr451) (ds RNA-dependent Protein Kinase); Anti -PKR; anti-pkr antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PKR when phosphorylated at Thr451. Species Sequence Homology: mouse, 73%
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
Applicable Applications for anti-pkr antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide corresponding to the region of human PKR that contains threonine 451.
Positive Control
HeLa cells stimulated with interferon alpha/beta (IFNa/b) and calyculin.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis using MBS631158.)
Western Blot (WB)
(Western Blot analysis using MBS631158.)
Related Product Information for anti-pkr antibody
Double-stranded RNA-dependent protein kinase (PKR) is a 68 kDa protein that is induced by interferon and double-stranded RNAs (dsRNA) produced in virus-infected cells. Two dsRNA- binding domains in the N-terminus interact with dsRNA to modify the conformation of PKR and allow it to undergo autophosphorylation and activation. Once activated, PKR phosphorylates eIF2alpha, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction. The phosphorylation of two sites in the activation loop of the kinase domain, threonines 446 and 451, is critical for high level catalytic activity.
Product Categories/Family for anti-pkr antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
LOW QUALITY PROTEIN: interferon-induced, double-stranded RNA-activated protein kinase
NCBI Official Symbol
pkr
NCBI Protein Information
double-stranded RNA activated protein kinase
Similar Products
Product Notes
The pkr (Catalog #AAA631158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKR, phosphorylated (Thr451) (ds RNA-dependent Protein Kinase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the pkr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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