Rabbit BANP Polyclonal Antibody | anti-BANP antibody

BANP Antibody

Cat. Num. AAA150064

• Gene Names: BANP; BEND1; SMAR1; SMARBP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: BANP Antibody is affinity chromatography purified via peptide column.
• Synonyms: BANP; Polyclonal Antibody; BANP Antibody; BEND1; SMAR1; SMARBP1; Protein BANP; BEN domain-containing protein 1; BTG3 associated nuclear protein; anti-BANP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: BANP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 519

• Applicable Applications for anti-BANP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: BANP antibody can be used for detection of BANP by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: BANP antibody was raised against a 14 amino acid synthetic peptide near the center of human BANP.
• Buffer: BANP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: BANP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BANP in mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of BANP in human kidney tissue with BANP antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of BANP in human kidney tissue with BANP antibody at 20 μg/mL.)

Related Product Information for anti-BANP antibody

BANP Antibody: BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-beta signaling. Furthermore, BANP can modulate NF-κB transactivation and can inhibit tumorigenesis by regulating NF-κB target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix.

NCBI and Uniprot Product Information

• NCBI GI #: 291084803
• NCBI GeneID: 54971
• NCBI Accession #: NP_001167014
• NCBI GenBank Nucleotide #: NM_001173543.1
• UniProt Accession #: Q8N9N5; Q96GJ7; Q9NWY1; A8MU25; A8MX25; B2RCF7; B4DNJ9; F5GZM0
• Molecular Weight: 55,502 Da
• NCBI Official Full Name: protein BANP isoform g
• NCBI Official Synonym Full Names: BTG3 associated nuclear protein
• NCBI Official Symbol: BANP
• NCBI Official Synonym Symbols: BEND1; SMAR1; SMARBP1
• NCBI Protein Information: protein BANP; BEN domain-containing protein 1; scaffold/matrix-associated region-1-binding protein
• UniProt Protein Name: Protein BANP
• Protein Family: Protein
• UniProt Gene Name: BANP
• UniProt Synonym Gene Names: BEND1; SMAR1
• UniProt Entry Name: BANP_HUMAN

NCBI Description

This gene encodes a protein that binds to matrix attachment regions. The protein forms a complex with p53 and negatively regulates p53 transcription, and functions as a tumor suppressor and cell cycle regulator. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

BANP: Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest. Belongs to the BANP/SMAR1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding; p53 binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; chromatin modification; negative regulation of protein catabolic process; cell cycle