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Western Blot (WB) (Western blot analysis of lysates from human liver, human kidney tissue (from left to right), using POU5F1B Antibody (C-term). MBS9204100 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Rabbit anti-Human POU5F1B Polyclonal Antibody | anti-POU5F1B antibody

POU5F1B Antibody (C-term)

Gene Names
POU5F1B; OTF3C; OTF3P1; OCT4PG1; OCT4-PG1; POU5F1P1; POU5F1P4; POU5FLC8; POU5FLC20
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POU5F1B; Polyclonal Antibody; POU5F1B Antibody (C-term); Putative POU domain; class 5; transcription factor 1B; Oct4-pg1; Octamer-binding protein 3-like; Octamer-binding transcription factor 3-like; anti-POU5F1B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POU5F1B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 292-326 amino acids from the C-terminal region of human POU5F1B.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
359
Applicable Applications for anti-POU5F1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from human liver, human kidney tissue (from left to right), using POU5F1B Antibody (C-term). MBS9204100 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from human liver, human kidney tissue (from left to right), using POU5F1B Antibody (C-term). MBS9204100 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-POU5F1B antibody
Shows weak transcriptional activator activity.
Product Categories/Family for anti-POU5F1B antibody
References
Takeda J.,et al.Nucleic Acids Res. 20:4613-4620(1992).
Marques A.C.,et al.PLoS Biol. 3:E357-E357(2005).
van Roozendaal K.E.P.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Zhao F.-Q.,et al.Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
Zhao S.,et al.Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38588
NCBI Official Full Name
putative POU domain, class 5, transcription factor 1B
NCBI Official Synonym Full Names
POU class 5 homeobox 1B
NCBI Official Symbol
POU5F1B
NCBI Official Synonym Symbols
OTF3C; OTF3P1; OCT4PG1; OCT4-PG1; POU5F1P1; POU5F1P4; POU5FLC8; POU5FLC20
NCBI Protein Information
putative POU domain, class 5, transcription factor 1B
UniProt Protein Name
Putative POU domain, class 5, transcription factor 1B
UniProt Gene Name
POU5F1B
UniProt Synonym Gene Names
OCT4PG1; OTF3C; OTF3P1; POU5F1P1; POU5FLC20; POU5FLC8
UniProt Entry Name
P5F1B_HUMAN

NCBI Description

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq, Apr 2009]

Uniprot Description

POU5F1B: Shows weak transcriptional activator activity. Belongs to the POU transcription factor family. Class- 5 subfamily.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent

Research Articles on POU5F1B

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Product Notes

The POU5F1B pou5f1b (Catalog #AAA9204100) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU5F1B Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POU5F1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the POU5F1B pou5f1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU5F1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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