Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 Recombinant Protein | b4GALNT2 recombinant protein

Recombinant Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2)

Cat. Num. AAA2031365

• Gene Names: B4GALNT2; B4GALT; GALGT2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Beta-1; 4-N-Acetyl Galactosaminyl Transferase 2; Recombinant Beta-1; 4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2); b4GALNT2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; TIATKTF LRPHKLMIML RSIREYYPDLTVIVADDSQK PLEIKDNHVE YYTMPFGKGW FAGRNLAISQ VTTKYVLWVDDDFLFNEETK IEVLVDVLEK TELDVVGGSV LGNVFQFKLL LEQSENGACLHKRMGFFQPL DGFPSCVVTS GVVNFFLAHT ERLQRVGFDP RLQRVAHSEFFIDGLGTLLV GSCPEVIIGH QSRSPVVDSE LAALEKTYNT YRSNTLTRVQFKLALHYFKN HLQCAA
• Sequence Length: 566

• Applicable Applications for b4GALNT2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.3kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Thr324~Ala566
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 109731790
• NCBI GeneID: 124872
• Molecular Weight: 54,203 Da
• NCBI Official Full Name: Beta-1,4-N-acetyl-galactosaminyl transferase 2
• NCBI Official Synonym Full Names: beta-1,4-N-acetyl-galactosaminyl transferase 2
• NCBI Official Symbol: B4GALNT2
• NCBI Official Synonym Symbols: B4GALT; GALGT2
• NCBI Protein Information: beta-1,4 N-acetylgalactosaminyltransferase 2
• UniProt Protein Name: Beta-1,4 N-acetylgalactosaminyltransferase 2
• Protein Family: Beta-1,4 N-acetylgalactosaminyltransferase
• UniProt Gene Name: B4GALNT2
• UniProt Synonym Gene Names: GALGT2
• UniProt Entry Name: B4GN2_HUMAN

NCBI Description

B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed 12678917]).[supplied by OMIM, Mar 2008]

Uniprot Description

B4GALNT2: Involved in the synthesis of the Sd(a) antigen (Sia- alpha2,3-[GalNAc-beta1,4]Gal-beta1,4-GlcNAc), a carbohydrate determinant expressed on erythrocytes, the colonic mucosa and other tissues. Transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. Belongs to the glycosyltransferase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: integral to membrane; integral to Golgi membrane

Molecular Function: acetylgalactosaminyltransferase activity

Biological Process: UDP-N-acetylglucosamine metabolic process; lipid glycosylation; protein amino acid glycosylation; negative regulation of cell-cell adhesion; UDP-N-acetylgalactosamine metabolic process