Testing Data
(THP-1 cells were stained with MBS608948 (filled histogram) or isotype control (open histogram).)
Mouse anti-Human CX3CR1 Monoclonal Antibody | anti-CX3CR1 antibody
CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) (PE)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 10ul labels 1x10e6. Validated using human peripheral blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. Add10ul of C8299-01F to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspended cells in F4568-10 for flow cytometry analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody.
Testing Data
(THP-1 cells were stained with MBS608948 (filled histogram) or isotype control (open histogram).)
Testing Data
(THP-1 cells were stained with MBS608948 (filled histogram) or isotype control (open histogram).)
NCBI and Uniprot Product Information
NCBI Description
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
CX3CR1: Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. Defects in CX3CR1 are a cause of susceptibility to age- related macular degeneration type 12 (ARMD12). ARMD12 is a form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion; Receptor, cytokine; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: neuron projection; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; chemokine receptor activity; C-X3-C chemokine receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of angiogenesis; cerebral cortex cell migration; viral reproduction; response to wounding; cellular defense response; chemotaxis; cell adhesion; macrophage chemotaxis; negative regulation of cell migration; microglial cell activation during immune response
Disease: Macular Degeneration, Age-related, 12; Coronary Heart Disease, Susceptibility To, 1; Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on CX3CR1
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Product Notes
The CX3CR1 cx3cr1 (Catalog #AAA608948) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CX3CR1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for Flow Cytometry. Dilution: Flow Cytometry: 10ul labels 1x10e6. Validated using human peripheral blood monocytes. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. Add10ul of C8299-01F to 1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspended cells in F4568-10 for flow cytometry analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody. Researchers should empirically determine the suitability of the CX3CR1 cx3cr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CX3CR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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