Human Galanin (GAL) CLIA Kit | GAL clia kit

Human Galanin (GAL) CLIA Kit

Cat. Num. AAA2707949

• Gene Names: GAL; ETL8; GALN; GLNN; GMAP; GAL-GMAP
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Galanin (GAL); Human Galanin (GAL) CLIA Kit; Galanin; GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide; GAL clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Galanin (GAL).; No significant cross-reactivity or interference between Galanin (GAL) and analogues was observed.
• Sequence Length: 123

• Applicable Applications for GAL clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 11.72-3,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 4.23pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Galanin (GAL). A competitive inhibition reaction is launched between biotin labeled Galanin (GAL) and unlabeled Galanin (GAL) (Standards or samples) with the pre-coated antibody specific to Galanin (GAL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Galanin (GAL) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Galanin (GAL) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 27804313
• NCBI GeneID: 51083
• NCBI Accession #: NP_057057.2
• NCBI GenBank Nucleotide #: NP_057057.2
• UniProt Accession #: P22466
• NCBI Official Full Name: galanin peptides preproprotein
• NCBI Official Synonym Full Names: galanin and GMAP prepropeptide
• NCBI Official Symbol: GAL
• NCBI Official Synonym Symbols: ETL8; GALN; GLNN; GMAP; GAL-GMAP
• NCBI Protein Information: galanin peptides
• UniProt Protein Name: Galanin peptides
• Protein Family: Galanin
• UniProt Gene Name: GAL
• UniProt Synonym Gene Names: GAL1; GALN; GLNN; GMAP
• UniProt Entry Name: GALA_HUMAN

NCBI Description

This gene encodes a neuroendocrine peptide that is widely expressed in the central and peripheral nervous systems and also the gastrointestinal tract, pancreas, adrenal gland and urogenital tract. The encoded protein is a precursor that is proteolytically processed to generate two mature peptides: galanin and galanin message-associated peptide (GMAP). Galanin has diverse physiological functions including nociception, feeding and energy homeostasis, osmotic regulation and water balance. GMAP has been demonstrated to possess antifungal activity and hypothesized to be part of the innate immune system. [provided by RefSeq, Jul 2015]

Uniprot Description

Galanin: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Belongs to the galanin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: Golgi apparatus; extracellular space; cell soma; extracellular region; secretory granule

Molecular Function: type 3 galanin receptor binding; neuropeptide hormone activity; protein binding; type 2 galanin receptor binding; type 1 galanin receptor binding

Biological Process: response to drug; nervous system development; positive regulation of cortisol secretion; positive regulation of apoptosis; positive regulation of catagen; negative regulation of lymphocyte proliferation; response to insulin stimulus; cAMP-mediated signaling; response to estrogen stimulus; insulin secretion; neuropeptide signaling pathway; regulation of glucocorticoid metabolic process; positive regulation of transcription from RNA polymerase II promoter; feeding behavior; inflammatory response

Disease: Epilepsy, Familial Temporal Lobe, 8