Rabbit anti-Human DPYSL3 Polyclonal Antibody | anti-DPYSL3 antibody

DPYSL3, CT (DPYSL3, CRMP4, DRP3, ULIP, ULIP1, Dihydropyrimidinase-related protein 3, Collapsin response mediator protein 4, Unc-33-like phosphoprotein 1) (PE)

Cat. Num. AAA6293465

• Gene Names: DPYSL3; DRP3; ULIP; CRMP4; DRP-3; LCRMP; CRMP-4; ULIP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DPYSL3; Polyclonal Antibody; CT (DPYSL3; CRMP4; DRP3; ULIP; ULIP1; Dihydropyrimidinase-related protein 3; Collapsin response mediator protein 4; Unc-33-like phosphoprotein 1) (PE); Unc-33-like phosphoprotein 1; anti-DPYSL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DPYSL3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DPYSL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-490 amino acids from the C-terminal region of human DPYSL3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DPYSL3 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DPYSL3 antibody detected the DPYSL3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of DPYSL3 (arrow) using rabbit polyclonal DPYSL3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DPYSL3 gene.)

Immunohistochemistry (IHC)

(DPYSL3 Antibody (C-term) (AP14234b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPYSL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DPYSL3 antibody

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).

Product Categories/Family for anti-DPYSL3 antibody

Antibodies; Enzymes

References

Okita, K., et al. Cancer Sci. 101(11):2333-2340(2010). Gao, X., et al. Oncogene 29(32):4555-4566(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4503379
• NCBI GeneID: 1809
• NCBI Accession #: NP_001378.1
• NCBI GenBank Nucleotide #: NM_001387.2
• UniProt Accession #: Q14195; Q93012; B3SXQ8
• Molecular Weight: 61,963 Da
• NCBI Official Full Name: dihydropyrimidinase-related protein 3 isoform 2
• NCBI Official Synonym Full Names: dihydropyrimidinase-like 3
• NCBI Official Symbol: DPYSL3
• NCBI Official Synonym Symbols: DRP3; ULIP; CRMP4; DRP-3; LCRMP; CRMP-4; ULIP-1
• NCBI Protein Information: dihydropyrimidinase-related protein 3; unc-33-like phosphoprotein 1; collapsin response mediator protein 4 long
• UniProt Protein Name: Dihydropyrimidinase-related protein 3
• Protein Family: Dihydropyrimidinase-related protein
• UniProt Gene Name: DPYSL3
• UniProt Synonym Gene Names: CRMP4; DRP3; ULIP; ULIP1; DRP-3; CRMP-4; ULIP-1
• UniProt Entry Name: DPYL3_HUMAN

Uniprot Description

CRMP-4: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: filamentous actin; extracellular space; growth cone; lamellipodium; cytosol

Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides; phosphoprotein binding; SH3 domain binding

Biological Process: actin crosslink formation; positive regulation of filopodium formation; axon guidance; actin filament bundle formation; response to axon injury; negative regulation of cell migration; pyrimidine base catabolic process; protein homooligomerization