Rabbit anti-Human POLR3B Polyclonal Antibody | anti-POLR3B antibody

Anti-POLR3B Antibody, Rabbit Polyclonal

Cat. Num. AAA8109663

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: POLR3B; Polyclonal Antibody; Anti-POLR3B Antibody; Rabbit Polyclonal; POLR3B Antibody; Rabbit PAb; Antigen Affinity Purified; anti-POLR3B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human POLR3B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-POLR3B antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200
• Immunogen: E Coli-derived Human POLR3B fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human POLR3B fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-POLR3B rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:128 kDa Observed band size:130 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human POLR3B in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-POLR3B antibody

POLR3A and POLR3B encode the largest and second largest subunits of RNA Polymerase III (Pol III), RPC1 and RPC2, respectively. RPC1 and RPC2 together form the active center of the polymerase and contribute to the catalytic activity of the polymerase. Pol III is involved in the transcription of small noncoding RNAs, such as 5S ribosomal RNA and all transfer RNAs (tRNA). Recessive mutations in the ubiquitously expressed POLR3A and POLR3B genes are the most common cause of POLR3-related hypomyelinating leukodystrophy (POLR3-HLD), a rare childhood-onset disorder characterized by deficient cerebral myelin formation and cerebellar atrophy.

References

Saitsu H, et al. (2011) Mutations in polr3a and polr3b encoding rna polymerase iii subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy. Am J Hum Genet 89 (5): 644-651.Richards MR, et al. (2017) Phenotypic spectrum of polr3b mutations: Isolated hypogonadotropic hypogonadism without neurological or dental anomalies. J Med Genet 54 (1): 19-25.Choquet K, et al. (2019) The leukodystrophy mutation polr3b r103h causes homozygote mouse embryonic lethality and impairs rna polymerase iii biogenesis. Mol Brain 12 (1): 59.