Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-tocopherol transfer protein (TTPA) Standard Curve.)
Mouse Alpha-Tocopherol Transfer Protein (TTPA) ELISA Kit | TTPA elisa kit
Mouse Alpha-Tocopherol Transfer Protein (TTPA) ELISA Kit
Gene Names
Ttpa; alpha-TTP
Reactivity
Mouse
Synonyms
Alpha-Tocopherol Transfer Protein (TTPA); Mouse Alpha-Tocopherol Transfer Protein (TTPA) ELISA Kit; TTPA; ATTP; AVED; TTP1; alphaTTP; TTPA elisa kit
Reactivity
Mouse
Specificity
Mouse Alpha-tocopherol transfer protein (TTPA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TTPA. No significant cross-reactivity or interference between Mouse TTPA and analogues was observed.
Sequence Length
278
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-tocopherol transfer protein (TTPA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-tocopherol transfer protein (TTPA) Standard Curve.)
Related Product Information for TTPA elisa kit
Description: This Mouse Alpha-tocopherol transfer protein (TTPA) ELISA Kit employs a two-site sandwich ELISA to quantitate TTPA in samples. An antibody specific for TTPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TTPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TTPa encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. Using rat alpha-Ttp to screen a liver cDNA library, followed by PCR, Arita et al. (1995) cloned full-length human alpha-TTP. The deduced 278-amino acid protein has a calculated molecular mass of 31.7 kD and shares 94% identity with rat alpha-Ttp. Northern blot analysis of several human tissues detected a 4.5-kb alpha-TTP transcript in liver only.
Background: TTPa encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. Using rat alpha-Ttp to screen a liver cDNA library, followed by PCR, Arita et al. (1995) cloned full-length human alpha-TTP. The deduced 278-amino acid protein has a calculated molecular mass of 31.7 kD and shares 94% identity with rat alpha-Ttp. Northern blot analysis of several human tissues detected a 4.5-kb alpha-TTP transcript in liver only.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-tocopherol transfer protein isoform 1
NCBI Official Synonym Full Names
tocopherol (alpha) transfer protein
NCBI Official Symbol
Ttpa
NCBI Official Synonym Symbols
alpha-TTP
NCBI Protein Information
alpha-tocopherol transfer protein
UniProt Protein Name
Alpha-tocopherol transfer protein
Protein Family
UniProt Gene Name
Ttpa
UniProt Synonym Gene Names
Alpha-TTP
UniProt Entry Name
TTPA_MOUSE
Uniprot Description
TTPA: Binds alpha-tocopherol and enhances its transfer between separate membranes. Defects in TTPA are the cause of ataxia with isolated vitamin E deficiency (AVED). AVED is an autosomal recessive disease characterized by spinocerebellar degeneration. It causes ataxia and peripheral neuropathy that resembles Friedreich ataxia. AVED patients have markedly reduced plasma levels of vitamin E.
Cellular Component: cytoplasm; cytosol; intracellular; late endosome
Molecular Function: lipid binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; transporter activity; vitamin binding; vitamin E binding
Biological Process: embryonic placenta development; intermembrane transport; intracellular pH reduction; placenta development; response to pH; response to toxin; transport; vitamin E metabolic process; vitamin transport
Research Articles on TTPA
Similar Products
Product Notes
The Mouse TTPA ttpa (Catalog #AAA9716659) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716659 ELISA Kit recognizes Mouse TTPA. It is sometimes possible for the material contained within the vial of "Alpha-Tocopherol Transfer Protein (TTPA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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